Human/Mouse/Rat PDCD5 Antibody

Catalog #: MAB7325 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7325
MAB7325-SP

Detection of Human, Mouse, and Rat PDCD5 by Western Blot.

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Human/Mouse/Rat PDCD5 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human PDCD5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human PDCD6 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 728906

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PDCD5
Ala2-Tyr125
Accession # O14737

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat PDCD5 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, EL-4 mouse lymphoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD5 Polyclonal Antibody (Catalog # MAB7325) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD5 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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PDCD5 in Human Stomach. PDCD5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human/Mouse/Rat PDCD5 Monoclonal Antibody (Catalog # MAB7325) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDCD5

PDCD5 (programmed cell death 5), also called TFAR19 (TF-1 cell apoptosis-related 19) is a ubiquitously expressed, approximately 15 kDa protein that is upregulated during apoptosis and downregulated in many cancer cell types. Of its 124 amino acids (aa), 44 are acidic or basic. PDCD5 also contains two acetylated aa and one constitutively phosphorylated aa, S119, which is involved in promoting apoptosis. Human PDCD5 shares 95% and 96% aa sequence identity with mouse and rat PDCD5, respectively.

Long Name

Programmed Cell Death 5

Entrez Gene IDs

9141 (Human); 56330 (Mouse); 292814 (Rat)

Alternate Names

FLJ42784; MGC9294; PD5; PDCD5; programmed cell death 5; programmed cell death protein 5; Protein TFAR19; TF-1 cell apoptosis-related protein 19; TFAR19 novel apoptosis-related; TFAR19; TFAR19TF1 cell apoptosis-related gene 19