Human/Mouse/Rat Peroxiredoxin 2 Antibody Summary
25% cross‑reactivity with recombinant human (rh) Peroxiredoxin 1 and rhPeroxiredoxin 4 is observed, approximately 10% cross-reactivity with rhPeroxiredoxin 3 is observed, and less than 1% cross‑reactivity with rhPeroxiredoxin 5 and rhPeroxiredoxin 6 is observed.
Met1-Asn198
Accession # P32119
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
View Larger
Detection of Human Peroxiredoxin 2 by Simple WesternTM. Simple Western shows lysates of Exosome Standard (PC‑3) (NBP2-49856) and Raji human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 2 at approximately 26 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 2
Human peroxiredoxin-2 (Prx-2 or PRDX2; also Thioredoxin Peroxidase 1) is a 22 kDa antioxidant enzyme that belongs to the typical 2-Cys class of the THP/ahpC family of proteins. The precursor molecule is 198 amino acids (aa) in length, and has two catalytic cysteines, one at Cys50 and a second at Cys171. Prx-2 is an obligate homodimer. Inactive, it is apparently noncovalently associated. Upon peroxide binding to Cys50 of subunit 1, the Cys171 of subunit 2 interacts with Cys50 of subunit 1 to complete the antioxidation, generating a disulfide bond between Cys50 and Cys171. Subsequent reduction restores the subunits to the basal state. There are two additional isoforms. Isoform-b shows a deletion of aa 36‑86, while isoform c shows a substitution of 56 aa after residue 86. Human Prx-2 is 93% aa identical to mouse and rat Prx-2.
Product Datasheets
Citations for Human/Mouse/Rat Peroxiredoxin 2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
Filter your results:
Filter by:
-
Mitochondrial H2O2 Generation Using a Tunable Chemogenetic Tool To Perturb Redox Homeostasis in Human Cells and Induce Cell Death
Authors: Kassi T. Stein, Sun Jin Moon, Hadley D. Sikes
ACS Synthetic Biology
-
Miro1-mediated mitochondrial positioning supports subcellular redox status
Authors: H Alshaabi, N Shannon, R Gravelle, S Milczarek, T Messier, B Cunniff
Redox Biology, 2020-11-29;38(0):101818.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Kinetic modeling of H2O2 dynamics in the mitochondria of HeLa cells
Authors: KT Stein, SJ Moon, AN Nguyen, HD Sikes
PLoS Comput. Biol., 2020-09-14;16(9):e1008202.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
A role for peroxiredoxins in H2O2- and MEKK-dependent activation of the p38 signaling pathway
Authors: AG Barata, TP Dick
Redox Biol, 2019-10-08;28(0):101340.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Peroxiredoxin-2 and STAT3 form a redox relay for H2O2 signaling.
Authors: Sobotta M, Liou W, Stocker S, Talwar D, Oehler M, Ruppert T, Scharf A, Dick T
Nat Chem Biol, 2014-11-24;11(1):64-70.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Reactive Oxygen Species Differentially Modulate the Metabolic and Transcriptomic Response of Endothelial Cells
Authors: Niklas Müller, Timothy Warwick, Kurt Noack, Pedro Felipe Malacarne, Arthur J. L. Cooper, Norbert Weissmann et al.
Antioxidants (Basel)
-
A role for 2-Cys peroxiredoxins in facilitating cytosolic protein thiol oxidation
Authors: S Stöcker, M Maurer, T Ruppert, TP Dick
Nat. Chem. Biol., 2017-12-18;0(0):.
-
A unique model for SDH- deficient GIST: an endocrine related cancer
Authors: James F. Powers, Brent Cochran, James D. Baleja, Hadley D. Sikes, Xue Zhang, Inna Lomakin et al.
Endocrine-Related Cancer
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse/Rat Peroxiredoxin 2 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse/Rat Peroxiredoxin 2 Antibody and earn rewards!
Have you used Human/Mouse/Rat Peroxiredoxin 2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
