Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Catalog # Availability Size / Price Qty
MAB2998
MAB2998-SP
Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha  by Western Blot.
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Product Details
Citations (3)
FAQs
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Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat PI 3-Kinase p85 alpha in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 572106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PI 3-Kinase p85 alpha
aa Lys519-Gly644
Accession # P27986
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PI 3-Kinase p85a antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, K562 human chronic myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3-Kinase p85a Monoclonal Antibody (Catalog # MAB2998) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85a at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human PI 3‑Kinase p85a antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human PI 3‑Kinase p85 alpha by Simple WesternTM. Simple Western lane view shows lysates of A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PI 3‑Kinase p85 alpha at approximately 89 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat PI 3‑Kinase p85 alpha Monoclonal Antibody (Catalog # MAB2998). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p85 alpha

PI 3-Kinases phosphorylate the inositol ring on a variety of membrane-associated phosphatidylinositols. Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adaptor, coupling catalytic p110 to activated receptor tyrosine kinases.

Long Name
PI 3-Kinase p85 alpha
Entrez Gene IDs
5295 (Human)
Alternate Names
AGM7; GRB1; IMD36; p85; p85-ALPHA; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI 3Kinase p85 alpha; PI 3-Kinase p85 alpha; PI3K regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-alpha

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Citations for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells
    Authors: Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al.
    Pharmaceuticals (Basel)
  2. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Gut microbiome catabolites as novel modulators of muscle cell glucose metabolism
    Authors: Michael J. Houghton, Asimina Kerimi, Vincent Mouly, Sarka Tumova, Gary Williamson
    The FASEB Journal

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