Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

Catalog #: AF2998
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2998
AF2998-SP

Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PI 3-Kinase p85 alpha in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PI 3-Kinase p85 alpha
Leu328-Tyr431
Accession # P27986

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3‑Kinase p85 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3‑Kinase p85 alpha at approximately 85 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p85 alpha

Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adapter, coupling catalytic p110 to activated receptor tyrosine kinases.

Long Name

PI 3-Kinase p85 alpha

Entrez Gene IDs

5295 (Human)

Alternate Names

AGM7; GRB1; IMD36; p85; p85-ALPHA; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI 3Kinase p85 alpha; PI 3-Kinase p85 alpha; PI3K regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PIK3R1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosis.
Authors: Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S
Exp. Cell Res., 2009-09-19;315(19):3301-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot