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Human/Mouse/Rat PTK7/CCK4 Antibody

Catalog #: AF4499
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4499
AF4499-SP
Detection of Human, Mouse, and Rat PTK7/CCK4 by Western Blot.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PTK7/CCK4 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat PTK7/CCK4 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat PTK7/CCK4 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat PTK7/CCK4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Rat-2 rat embryonic fibroblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTK7/CCK4 at approximately 160 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western Detection of Human PTK7/CCK4 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human PTK7/CCK4 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTK7/CCK4 at approximately 171 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PTK7/CCK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4499) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTK7/CCK4

CCK4 (colon carcinoma kinase 4, also known as PTK7) is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, CCK4 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner.

Long Name
Protein Tyrosine Kinase 7
Entrez Gene IDs
5754 (Human); 71461 (Mouse); 301242 (Rat)
Alternate Names
CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; PTK7; Tyrosine-protein kinase-like 7

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Citations for Human/Mouse/Rat PTK7/CCK4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Protein tyrosine kinase 7 (PTK7) promotes metastasis in hepatocellular carcinoma via SOX9 regulation and TGF-beta signaling
    Authors: MT Lam Wong, TL Wong, L Zhou, K Man, J Purcell, TK Lee, JP Yun, S Ma
    Cellular and Molecular Gastroenterology and Hepatology, 2022-10-04;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Quantitative Proteomics Reveals Changes Induced by TIMP-3 on Cell Membrane Composition and Novel Metalloprotease Substrates
    Authors: Anna Paola Carreca, Veronica Maria Pravatà, Danilo D’Apolito, Simone Bonelli, Matteo Calligaris, Elisa Monaca et al.
    International Journal of Molecular Sciences
  3. The Lipid Receptor G2A (GPR132) Mediates Macrophage Migration in Nerve Injury-Induced Neuropathic Pain
    Authors: T Osthues, B Zimmer, V Rimola, K Klann, K Schilling, P Mathoor, C Angioni, A Weigert, G Geisslinge, C Münch, K Scholich, M Sisignano
    Cells, 2020-07-21;9(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Protein-tyrosine pseudokinase 7 (PTK7) directs cancer cell motility and metastasis.
    Authors: Golubkov V, Prigozhina N, Zhang Y, Stoletov K, Lewis J, Schwartz P, Hoffman R, Strongin A
    J Biol Chem, 2014-07-08;289(35):24238-49.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Downstream signaling and genome-wide regulatory effects of PTK7 pseudokinase and its proteolytic fragments in cancer cells.
    Authors: Golubkov V, Strongin A
    Cell Commun Signal, 2014-03-11;12(0):15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Insights into ectodomain shedding and processing of protein-tyrosine pseudokinase 7 (PTK7).
    Authors: Golubkov V, Strongin A
    J Biol Chem, 2012-10-24;287(50):42009-18.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Western Blot
  7. Potential relation of aberrant proteolysis of human protein tyrosine kinase 7 (PTK7) chuzhoi by membrane type 1 matrix metalloproteinase (MT1-MMP) to congenital defects.
    Authors: Golubkov VS, Aleshin AE, Strongin AY
    J. Biol. Chem., 2011-04-25;286(23):20970-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Flt-1 regulates vascular endothelial cell migration via a protein tyrosine kinase-7-dependent pathway.
    Authors: Lee HK, Chauhan SK, Kay E
    Blood, 2011-04-01;117(21):5762-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene.
    Authors: Fontanals-Cirera B, Hasson D, Vardabasso C et al.
    Mol Cell

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