Human/Mouse RBP4/Retinol-Binding Protein 4 Antibody

Catalog #: MAB34762 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB34762
MAB34762-SP

Detection of Human RBP4/Retinol‑Binding Protein 4 by Western Blot.

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Human/Mouse RBP4/Retinol-Binding Protein 4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse RBP4/Retinol‑Binding Protein 4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human RBP4 is observed.

Source

Monoclonal Rat IgG_2A Clone # 423614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RBP4/Retinol‑Binding Protein 4
Glu19-Leu201
Accession # Q00724

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Human liver tissue, human serum, and Recombinant Mouse RBP4/Retinol-Binding Protein 4 (Catalog # 3476-LC) at 0.1-1 μg/mL concentration of MAB34762

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse RBP4/Retinol-Binding Protein 4 (Catalog # 3476-LC), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human RBP4/Retinol‑Binding Protein 4 by Western Blot. Western blot shows lysates of human liver tissue, and human serum. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Human/Mouse RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB34762) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with Retinol-binding Protein 4 (RBP4), also known as Plasma Retinol-binding Protein (1‑3). A prototypic member of the lipocalin superfamily, RBP4 has a beta ‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases. The amino acid sequence of mouse RBP4 is 99%, 86%, 83% and 75% identical to that of rat, human/chimpanzee, dog and chicken.

References