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Human/Mouse SEZ6L2/BSRP-A Antibody

Catalog #: AF4916
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4916
AF4916-SP
SEZ6L2/BSRP-A in Mouse Embryo.
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Citations (6)
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Human/Mouse SEZ6L2/BSRP-A Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
Leu28-Ser837
Accession # Q4V9Z5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SEZ6L2/BSRP-A
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry SEZ6L2/BSRP-A antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

SEZ6L2/BSRP-A in Mouse Embryo. SEZ6L2/BSRP-A was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse SEZ6L2/BSRP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4916) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (medulla oblongata), spinal cord, trigeminal ganglia, and retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEZ6L2/BSRP-A

SEZ6L2 (Brain-specific receptor-like protein A; also BSRP-A) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and likely regulates PKC alpha activation. Mature mouse SEZ6L2 is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for SEZ6L2. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse SEZ6L2 is 96% and 99% aa identical to human and rat SEZ6L2, respectively.

Long Name
Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A
Entrez Gene IDs
26470 (Human); 233878 (Mouse); 308988 (Rat)
Alternate Names
BSRPA; BSRP-A; PSK-1; seizure 6-like protein 2; seizure related 6 homolog (mouse)-like 2; SEZ6L2; UNQ1903/PRO4349

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Citations for Human/Mouse SEZ6L2/BSRP-A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3
    Authors: Martina Pigoni, Hung‐En Hsia, Jana Hartmann, Jasenka Rudan Njavro, Merav D Shmueli, Stephan A Müller et al.
    The EMBO Journal
  2. The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases
    Authors: Wen Q. Qiu, Shaopeiwen Luo, Stefanie A. Ma, Priyanka Saminathan, Herman Li, Jenny M. Gunnersen et al.
    Frontiers in Immunology
  3. BACE inhibitor treatment of mice induces hyperactivity in a Seizure-related gene 6 family dependent manner without altering learning and memory
    Authors: A Nash, HJM Gijsen, BJ Hrupka, KS Teng, SF Lichtentha, H Takeshima, JM Gunnersen, KM Munro
    Scientific Reports, 2021-07-23;11(1):15084.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice
    Authors: A Shahapal, EB Cho, HJ Yong, I Jeong, H Kwak, JK Lee, W Kim, B Kim, HC Park, WS Lee, H Kim, JI Hwang, JY Seong
    Front Neurosci, 2019-08-30;13(0):917.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Cathepsin D and its newly identified transport receptor Sez6l2 can modulate neurite outgrowth
    Authors: Marielle Boonen, Catherine Staudt, Florentine Gilis, Viola Oorschot, Judith Klumperman, Michel Jadot
    Journal of Cell Science
  6. Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons
    Authors: Martina Pigoni, Johanna Wanngren, Peer-Hendrik Kuhn, Kathryn M. Munro, Jenny M. Gunnersen, Hiroshi Takeshima et al.
    Molecular Neurodegeneration

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