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Human/Mouse SEZ6L2/BSRP-A Biotinylated Antibody

Catalog #: BAF4916 Datasheet / COA / SDS
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BAF4916
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Human/Mouse SEZ6L2/BSRP-A Biotinylated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SEZ6L2/BSRP-A in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
Leu28-Ser837
Accession # Q4V9Z5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SEZ6L2/BSRP-A

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SEZ6L2/BSRP-A

SEZ6L2 (Brain-specific receptor-like protein A; also BSRP-A) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and likely regulates PKC alpha activation. Mature mouse SEZ6L2 is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for SEZ6L2. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse SEZ6L2 is 96% and 99% aa identical to human and rat SEZ6L2, respectively.

Long Name
Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A
Entrez Gene IDs
26470 (Human); 233878 (Mouse); 308988 (Rat)
Alternate Names
BSRPA; BSRP-A; PSK-1; seizure 6-like protein 2; seizure related 6 homolog (mouse)-like 2; SEZ6L2; UNQ1903/PRO4349

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