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Human/Mouse SOX2 APC-conjugated Antibody

Catalog #: IC2018A
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC2018A
Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SOX2 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SOX2 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 245610
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of SOX2 antibody in NTera‑2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SOX2 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SOX2 APC-conjugated Monoclonal Antibody (Catalog # IC2018A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).

References
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name
Transcription Factor SOX2
Entrez Gene IDs
6657 (Human)
Alternate Names
ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2

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Citations for Human/Mouse SOX2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Systematic optimization of culture media for maintenance of human induced pluripotent stem cells using the response surface methodology
    Authors: Mousavi Mirkalaei, S;Farivar, S;
    Heliyon
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The STEMRI trial: Magnetic resonance spectroscopy imaging can define tumor areas enriched in glioblastoma stem-like cells
    Authors: Lemarié, A;Lubrano, V;Delmas, C;Lusque, A;Cerapio, JP;Perrier, M;Siegfried, A;Arnauduc, F;Nicaise, Y;Dahan, P;Filleron, T;Mounier, M;Toulas, C;Cohen-Jonathan Moyal, E;
    Science advances
    Species: Human
    Sample Types: Whole Tissue
    Applications: Flow Cytometry
  3. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells.
    Authors: Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, Song J, de Vries H, Badhai J, Sutherland K, Krimpenfort P, Michalak E, Jonkers J, Berns A
    EMBO Mol Med, 2014-01-08;6(2):212-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Chemokine receptor CXCR3 promotes growth of glioma.
    Authors: Liu C, Luo D, Reynolds BA
    Carcinogenesis, 2010-11-03;32(2):129-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IGF-1 contributes to the expansion of melanoma-initiating cells through an epithelial-mesenchymal transition process
    Authors: Vincent Le Coz, Chaobin Zhu, Aurore Devocelle, Aimé Vazquez, Claude Boucheix, Sandy Azzi et al.
    Oncotarget

FAQs

  1. What is the light chain of Human/Mouse/Rat SOX2 Antibody, Catalog #s MAB2018, MAB2018R and IC2018?

    • Catalog #s MAB2018, MAB2018R and IC2018 all have a kappa light chain.

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