Home / SSEA-1 / Human/Mouse SSEA-1 APC-conjugated Antibody

Human/Mouse SSEA-1 APC-conjugated Antibody

Catalog #: FAB2155A
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2155A-100
FAB2155A-025
Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-1 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SSEA-1.
Source
Monoclonal Mouse IgM Clone # MC-480
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
F9 mouse teratocarcinoma stem cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µL/106 cells
D3 mouse embryonic stem cell line and whole blood granulocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-1 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 APC-conjugated Monoclonal Antibody (Catalog # FAB2155A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of SSEA‑1 in Whole blood granulocytes by Flow Cytometry. Whole blood granulocytes were stained with Mouse Anti-Human/Mouse SSEA‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB2155A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References
  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1

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Citations for Human/Mouse SSEA-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Deep annotation of mouse iso-miR and iso-moR variation
    Authors: Hongjun Zhou, Mary Luz Arcila, Zhonghan Li, Eun Joo Lee, Christine Henzler, Jingya Liu et al.
    Nucleic Acids Research
  2. FANCD2 is required for the repression of germline transposable elements
    Authors: Yan Nie, Andrew F. Wilson, Tony DeFalco, Amom Ruhikanta Meetei, Satoshi H Namekawa, Qishen Pang
    Reproduction
  3. Homologous Recombination DNA Repair Genes Play a Critical Role in Reprogramming to a Pluripotent State
    Authors: Federico González, Daniela Georgieva, Fabio Vanoli, Zhong-Dong Shi, Matthias Stadtfeld, Thomas Ludwig et al.
    Cell Reports
  4. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019-11-04;10(1):316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch
    Sci Rep, 2016-08-02;6(0):30903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  6. Zrf1 is required to establish and maintain neural progenitor identity.
    Authors: Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah S, Graf T, Broccoli V, Di Croce L
    Genes Dev, 2014-01-15;28(2):182-97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease.
    Authors: Griffiths DS, Li J, Dawson MA, Trotter MW, Cheng YH, Smith AM, Mansfield W, Liu P, Kouzarides T, Nichols J, Bannister AJ, Green AR, Gottgens B
    Nat. Cell Biol., 2010-12-12;13(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells.
    Authors: Brambrink T, Foreman R, Welstead GG, Lengner CJ, Wernig M, Suh H, Jaenisch R
    Cell Stem Cell, 2008-02-07;2(2):151-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues
    Authors: Eran Altman, Pamela Yango, Radwa Moustafa, James F. Smith, Peter C. Klatsky, Nam D. Tran
    REPRODUCTION

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