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Human/Mouse TIMP-2 Antibody

Catalog #: AF971
14 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF971
AF971-SP
Detection of Human TIMP‑2 by Western Blot.
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Product Details
Citations (14)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TIMP-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse TIMP‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human TIMP-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2
Cys27-Pro220
Accession # P16035
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Dual RNAscope ISH-IHC
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human placenta
Knockout Validated
TIMP‑2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in TIMP‑2 knockout HeLa human cervical epithelial carcinoma cell line.
 
Neutralization
Measured by its ability to neutralize Recombinant Human TIMP-2 (0.143 µg/mL, Catalog # 971-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MPN or 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL‑Dpa‑AR‑NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 2.6 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TIMP-2 antibody by Western Blot. View Larger

Detection of Human TIMP‑2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TIMP‑2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

TIMP‑2 in Human Ovarian Cancer Tissue. TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated View Larger

Western Blot Shows Human TIMP‑2 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human TIMP-2 knockout HeLa human cervical epithelial carcinoma cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated) in the parental HeLa human cervical epithelial carcinoma cell line, but is not detectable in knockout HeLa human cervical epithelial carcinoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Neutralization Neutralization of TIMP‑2 Activity by Human TIMP‑2 Antibody. View Larger

Neutralization of TIMP‑2 Activity by Human TIMP‑2 Antibody. Recombinant Human MMP-2 (0.2 µg/mL, Catalog # 902-MPNor 902-MP) activity is measured in the presence of Recombinant Human/Mouse TIMP-2 (0.143 µg/mL, Catalog # 971-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971). The ND50 is typically 2.6 µg/mL.

In-situ Hybridization View Larger

Detection of TIMP‑2 in Human Placenta. Formalin-fixed paraffin-embedded tissue sections of human placenta were probed for TIMP2 mRNA (ACD RNAScope Probe, catalog #470358; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human TIMP2 polyclonal antibody (R&D Systems catalog # AF971) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to decidual cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-2

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.

Long Name
Tissue Inhibitors of Metalloproteinases 2
Entrez Gene IDs
7077 (Human); 21858 (Mouse); 29543 (Rat)
Alternate Names
CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2

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Citations for Human/Mouse TIMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis
    Authors: Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al.
    Frontiers in Oncology
  2. Co-chaperones TIMP2 and AHA1 Competitively Regulate Extracellular HSP90:Client MMP2 Activity and Matrix Proteolysis
    Authors: Baker-Williams AJ, Hashmi F, Budzy?ski MA et al.
    Cell Reports
  3. Unique Transcriptional Programs Identify Subtypes of AKI
    Authors: Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen et al.
    Journal of the American Society of Nephrology
  4. Heroin Seeking and Extinction From Seeking Activate Matrix Metalloproteinases at Synapses on Distinct Subpopulations of Accumbens Cells
    Authors: Vivian C. Chioma, Anna Kruyer, Ana-Clara Bobadilla, Ariana Angelis, Zachary Ellison, Ritchy Hodebourg et al.
    Biological Psychiatry
  5. TIMP-2 binding with cellular MT1-MMP stimulates invasion-promoting MEK/ERK signaling in cancer cells
    Authors: Nor Eddine Sounni, Dmitri V. Rozanov, Albert G. Remacle, Vladislav S. Golubkov, Agnes Noel, Alex Y. Strongin
    International Journal of Cancer
  6. Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells
    Authors: Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Co-chaperones TIMP2 and AHA1 Competitively Regulate Extracellular HSP90:Client MMP2 Activity and Matrix Proteolysis
    Authors: Baker-Williams AJ, Hashmi F, Budzy?ski MA et al.
    Cell Reports
  8. Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction
    Authors: D Lu, Y Liao, SH Zhu, QC Chen, DM Xie, JJ Liao, X Feng, MH Jiang, W He
    Stem Cell Res Ther, 2019-04-27;10(1):127.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice
    Authors: JM Castellano, KI Mosher, RJ Abbey, AA McBride, ML James, D Berdnik, JC Shen, B Zou, XS Xie, M Tingle, IV Hinkson, MS Angst, T Wyss-Coray
    Nature, 2017-04-19;544(7651):488-492.
    Species: Human, Mouse
    Sample Types: In Vivo, Plasma, Whole Tissue
    Applications: Functional Assay, IHC-Fr, Immunoprecipitation, Neutralization
  10. Mitral tissue inhibitor of metalloproteinase 2 is associated with mitral valve surgery outcome.
    Authors: Lin T, Yang S, Chiu C, Su H, Voon W, Chai C, Lai W, Sheu S
    PLoS ONE, 2014-01-27;9(1):e86287.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Inhibition of airway epithelial-to-mesenchymal transition and fibrosis by kaempferol in endotoxin-induced epithelial cells and ovalbumin-sensitized mice.
    Authors: Gong J, Cho I, Shin D, Han S, Park S, Kang Y
    Lab Invest, 2013-12-30;94(3):297-308.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2008-09-14;145(1):42-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
    Authors: Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
    J. Cell Biol., 2006-10-09;175(1):179-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Fibroblasts repair blood-brain barrier damage and hemorrhagic brain injury via TIMP2
    Authors: Xu L, Nirwane A, Xu T et al.
    Cell reports

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Reviews for Human/Mouse TIMP-2 Antibody

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Human/Mouse TIMP-2 Antibody
By Anonymous on 02/16/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse

Dilution 1:300

Immunocytochemistry/Immunofluorescence Human/Mouse TIMP-2 Antibody AF971
Human/Mouse TIMP-2 Antibody
By Ruhul Choudhury on 01/23/2017
Application: IHC Sample Tested: Human first trimester decidua Species: Goat

sodium citrate antigen retrieval

Immunohistochemistry Human/Mouse TIMP-2 Antibody AF971