Human MRP1 Antibody

Name: Human MRP1 Antibody
Brand: R&D Systems
SKU: MAB19291
Rating: 5 (1 reviews)

Catalog #: MAB19291
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19291
MAB19291-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MRP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MRP1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # QCRL

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

H69AR human small cell lung cancer cell line membrane extracts

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Hipfner, D.R. et al. (1994) Canc. Res. 54:5788; Hipfner, D.R. et al. (1996) Canc. Res. 56:3307. This application was not tested by R&D Systems.

Immunoprecipitation

Hipfner, D.R., et al. (1994) Canc. Res. 54:5788.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed H69AR human small cell lung cancer cell line

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

H69AR human small cell lung cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MRP1 in Human PBMCs. MRP1 was detected in immersion fixed Human peripheral blood mononuclear cells using Mouse Anti-Human MRP1 Monoclonal Antibody (Catalog # MAB19291) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MRP1

MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.

Long Name

Multi-drug Resistance Protein 1

Entrez Gene IDs

4363 (Human); 17250 (Mouse); 24565 (Rat)

Alternate Names

ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 3.6.3.44; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human MRP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Molecular and biologic characterization and drug sensitivity of pan-histone deacetylase inhibitor-resistant acute myeloid leukemia cells.
Authors: Fiskus W, Rao R, Fernandez P, Herger B, Yang Y, Chen J, Kolhe R, Mandawat A, Wang Y, Joshi R, Eaton K, Lee P, Atadja P, Peiper S, Bhalla K
Blood, 2008-07-25;112(7):2896-905.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry