Human MRP1 Fluorescein-conjugated Antibody

Name: Human MRP1 Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: IC19291F

Catalog #: IC19291F
1 Citation Datasheet / COA / SDS

Discontinued Product

IC19291F has been discontinued.

View all MRP1 products.

Detection of MRP1 in H69AR Human Cell Line by Flow Cytometry.

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Citations (1)

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Human MRP1 Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MRP1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # QCRL

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

H69AR human small cell lung cancer cell line membrane extracts

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of MRP1 antibody in H69AR Human Cell Line antibody by Flow Cytometry.

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Detection of MRP1 in H69AR Human Cell Line by Flow Cytometry. H69AR human small cell lung cancer cell line was stained with Mouse Anti-Human MRP1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC19291F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MRP1

MRP1 (Multidrug Resistance associated Protein 1), also known as ABCC1, belongs to the family of ATP-Binding Cassette (ABC) transporters. It confers multidrug resistance on tumor cells and is overexpressed in various human multidrug resistant tumor cell lines.

Long Name

Multi-drug Resistance Protein 1

Entrez Gene IDs

4363 (Human); 17250 (Mouse); 24565 (Rat)

Alternate Names

ABC29; ABCC; ABCC1; ATP-binding cassette sub-family C member 1; ATP-binding cassette, sub-family C (CFTR/MRP), member 1; EC 3.6.3; EC 3.6.3.44; GS-X; Leukotriene C(4) transporter; LTC4 transporter; MRP1; MRP1DKFZp781G125; MRPDKFZp686N04233; multidrug resistance associated protein 1; multidrug resistance protein; multidrug resistance-associated protein 1; multiple drug resistance protein 1; multiple drug resistance-associated protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human MRP1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

MDR1 expression identifies human melanoma stem cells.
Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
Biochem. Biophys. Res. Commun., 2008-02-13;368(4):930-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry