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Human MSPR/Ron Antibody

Catalog #: AF691
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF691
AF691-SP
Detection of Human MSP R/Ron by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MSPR/Ron Antibody Summary

Species Reactivity
Human
Specificity
Delects human MSP R/Ron in direct ELISAs and Western blots. In these formats, approximately 25% cross‑reactivity with recombinant mouse MSP R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MSP R
Glu25-Ser956
Accession # CAA49634
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
1 µg/1 mg cell lysate
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human MSP (Catalog # 352-MS) to immobilized Recombinant Human MSP R/Ron (Catalog # 1947-MS) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MSP R/Ron antibody by Western Blot. View Larger

Detection of Human MSP R/Ron by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MSP R/Ron at approximately 150-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunoprecipitation Detection of Human MSP R/Ron by Immunoprecipitation. View Larger

Detection of Human MSP R/Ron by Immunoprecipitation. Human MSP R/Ron was immunoprecipitated from 1 mg of lysates of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691). The MSP R/Ron-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron was detected by Western blot using 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSP R cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25 - Arg309) and a 1091 aa residue transmembrane beta chain (Gly310 - Thr1400). The extracellular domain of MSP R is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSP R-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSP R, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSP R associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSP R and its associated transmembrane receptors have been demonstrated (6 - 8). Human MSP R shares 72% amino acid sequence identity with mouse MSP R.

References
  1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
  2. Wang, M-H. et al. (1994) Science 266:117.
  3. Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
  4. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
  5. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  6. Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
  7. Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
  8. Santora, M. et al. (2003) Devel. Cell 2:257.
Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

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Citations for Human MSPR/Ron Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
    Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
    The Journal of Biological Chemistry, 2022-05-13;0(0):102029.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  2. Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele.
    Authors: Kauder, Steven E, Santell, Lydia, Mai, Elaine, Wright, Lilyan Y, Luis, Elizabet, N'Diaye, Elsa N, Lutman, Jeff, Ratti, Navneet, Sa, Susan M, Maun, Henry R, Stefanich, Eric, Gonzalez, Lino C, Graham, Robert R, Diehl, Lauri, Faubion, William, Keir, Mary E, Young, Judy, Chaudhuri, Amitabha, Lazarus, Robert A, Egen, Jackson
    PLoS ONE, 2013-12-23;8(12):e83958.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Transactivation of RON receptor tyrosine kinase by interaction with PDGF receptor beta during steady-state growth of human mesangial cells.
    Authors: Kobayashi T, Furukawa Y, Kikuchi J, Ito C, Miyata Y, Muto S, Tanaka A, Kusano E
    Kidney Int., 2009-02-25;75(11):1173-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. EGFR transactivates RON to drive oncogenic crosstalk
    Authors: Carolina Franco Nitta, Ellen W Green, Elton D Jhamba, Justine M Keth, Iraís Ortiz-Caraveo, Rachel M Grattan et al.
    eLife

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