Human MSPR/Ron Biotinylated Antibody

Catalog #: BAF691 Datasheet / COA / SDS

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BAF691

All MSPR/Ron Antibodies

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Human MSPR/Ron Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MSP R in Western blots. In this format, approximately 25% cross-reactivity with recombinant mouse MSP R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MSP R
Glu25-Ser956
Accession # CAA49634

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MSP R/Ron (Catalog # 1947-MS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSP R cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25 - Arg309) and a 1091 aa residue transmembrane beta chain (Gly310 - Thr1400). The extracellular domain of MSP R is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSP R-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSP R, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSP R associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSP R and its associated transmembrane receptors have been demonstrated (6 - 8). Human MSP R shares 72% amino acid sequence identity with mouse MSP R.

References

Gaudino, G. et al. (1994) EMBO J. 13:3524.
Wang, M-H. et al. (1994) Science 266:117.
Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
Santora, M. et al. (2003) Devel. Cell 2:257.

Long Name

Macrophage Stimulating Protein Receptor

Entrez Gene IDs

4486 (Human); 19882 (Mouse)

Alternate Names

CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4