Human MUC-4 APC-conjugated Antibody

Catalog #: FAB8195A Datasheet / COA / SDS

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FAB8195A

Detection of MUC‑4 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human MUC-4 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MUC-4 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 781631

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MUC-4
Pro1072-Ser1317
Accession # Q99102

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MUC‑4 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human MUC-4 APC-conjugated Monoclonal Antibody (Catalog # FAB8195A, filled histogram) or isotype control antibody (IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MUC-4

MUC-4 (Mucin-4), also called ASGP (ascites sialoglycoprotein) is a highly glycosylated type I transmembrane glycoprotein that may be up to 950 kDa in its full-length, fully glycosylated form. Human MUC-4 cDNA encodes 2169 amino acids (aa) with a 28 aa signal sequence and a cleavage site that creates a 1416 aa soluble, extracellular alpha chain and a 725 aa single-pass transmembrane beta chain. Between aa 1072-1317 within the alpha chain, human MUC-4 shares 69% aa sequence identity with mouse and rat MUC-4. At least 14 soluble or transmembrane splice variants of 1102-2117 aa have been described, 5 of which contain the full sequence used as an immunogen. MUC-4 can serve as a ligand for the oncogenic receptor ErbB2 and a modulator of its phosphorylation and signaling. MUC-4 is frequently aberrantly expressed in epithelial tumors and can promote tumor growth and metastasis.

Long Name

Mucin 4, Cell Surface-associated

Entrez Gene IDs

4585 (Human); 140474 (Mouse)

Alternate Names

Ascites sialoglycoprotein; ASGP; HSA276359; MUC4; MUC-4; mucin 4, cell surface associated; mucin 4, tracheobronchial; mucin-4; Pancreatic adenocarcinoma mucin; Testis mucin; Tracheobronchial mucin