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Human Musashi-1 Antibody

Catalog #: MAB2628
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2628
MAB2628-SP
Detection of Human Musashi-1 by Western Blot.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Musashi-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Musashi-1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 282613
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Musashi-1
aa 1-362
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Musashi-1 antibody by Western Blot. View Larger

Detection of Human Musashi-1 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human Musashi-1 Monoclonal Antibody (Catalog # MAB2628) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Musashi-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Musashi-1

Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).

References
  1. Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
  2. Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.
Entrez Gene IDs
4440 (Human)
Alternate Names
MSI1; Musashi (Drosophila) homolog 1; musashi homolog 1 (Drosophila); Musashi1; Musashi-1; RNA-binding protein Musashi homolog 1

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Citations for Human Musashi-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Isolation and characterization of neural crest-like progenitor cells in human umbilical cord blood
    Authors: Z Al-Bakri, M Ishige-Wad, N Fukuda, C Yoshida-No, N Nagoshi, H Okano, H Mugishima, T Matsumoto
    Regenerative Therapy, 2020-07-18;15(0):53-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Integrin ?7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma
    Authors: TL Haas, MR Sciuto, L Brunetto, C Valvo, M Signore, ME Fiori, S di Martino, S Giannetti, L Morgante, A Boe, M Patrizii, U Warnken, M Schnölzer, A Ciolfi, C Di Stefano, M Biffoni, L Ricci-Viti, R Pallini, R De Maria
    Cell Stem Cell, 2017-06-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Isolation of cancer stem cells from three human glioblastoma cell lines: characterization of two selected clones.
    Authors: Iacopino F, Angelucci C, Piacentini R, Biamonte F, Mangiola A, Maira G, Grassi C, Sica G
    PLoS ONE, 2014-08-14;9(8):e105166.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Pharmacological inhibition of poly(ADP-ribose) polymerase-1 modulates resistance of human glioblastoma stem cells to temozolomide.
    Authors: Tentori, Lucio, Ricci-Vitiani, Lucia, Muzi, Alessia, Ciccarone, Fabio, Pelacchi, Federica, Calabrese, Roberta, Runci, Daniele, Pallini, Roberto, Caiafa, Paola, Graziani, Grazia
    BMC Cancer, 2014-03-05;14(0):151.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Identification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis.
    Authors: Chan RW, Ng EH, Yeung WS
    Am. J. Pathol., 2011-06-01;178(6):2832-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Aurora-A Inhibition Offers a Novel Therapy Effective against Intracranial Glioblastoma
    Authors: James R. Van Brocklyn, Jeffrey Wojton, Walter H. Meisen, David A. Kellough, Jeffery A. Ecsedy, Balveen Kaur et al.
    Cancer Research

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