Human Myosin Heavy Chain APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Myosin Heavy Chain in C2C12 cells by Flow Cytometry C2C12 cells were stained with Mouse Anti-Human Myosin Heavy Chain APC‑conjugated Monoclonal Antibody (Catalog # IC4470A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Myosin Heavy Chain
Skeletal muscle Myosin or Myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of Actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy Meromyosin, which includes the S1 motor domain (head region) and first third of the coiled-coil domain, and light Meromyosin, which includes the C-terminal two thirds of the coiled-coil domain.
Product Datasheets
Citation for Human Myosin Heavy Chain APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
An automated and high-throughput-screening compatible pluripotent stem cell-based test platform for developmental and reproductive toxicity assessment of small molecule compounds
Authors: Gesa Witt, Oliver Keminer, Jennifer Leu, Rashmi Tandon, Ina Meiser, Anne Willing et al.
Cell Biology and Toxicology
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Myosin Heavy Chain APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human Myosin Heavy Chain APC-conjugated Antibody and earn rewards!
Have you used Human Myosin Heavy Chain APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
