Human NAC1 Antibody

Catalog #: AF8375 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8375
AF8375-SP

Detection of Human NAC1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NAC1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NAC1 in direct ELISAs and Western Blots. In direct ELISAs, appoximately 100% cross-reactivity with recombinant mouse NAC1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NAC1
Val471-Gln527
Accession # Q96RE7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Knockout Validated

NAC1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NAC1 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NAC1 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAC1 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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NAC1 in Human Cervical Cancer Tissue. NAC1 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Sheep Anti-Human NAC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8375) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Knockout Validated

Western Blot Shows Human NAC1 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human NAC1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NAC1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAC1 at approximately 68 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAC1

NAC1 (nucleus accumbens-associated protein 1; also NACC1) is a 527 aa BTB/POZ domain containing protein. Human NAC1 shares 86% and 85% aa identity with mouse and rat NAC1, respectively. It has been shown to have a role in transcriptional regulation in both pluripotent stem cells and in the brain. Additionally, NAC1 expression is upregulated in many cancers and this expression has been suggested to correlate with increased aggressiveness in some cancers.

Long Name

Nucleus Accumbens Associated 1

Entrez Gene IDs

112939 (Human); 66830 (Mouse); 171454 (Rat)

Alternate Names

BEND8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; BTBD14B; BTBD30; NAC1; NAC1BEN domain containing 8; NAC-1FLJ37383; NACC1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; nucleus accumbens-associated protein 1; transcriptional repressor NAC1