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Human Nestin PE-conjugated Antibody

Catalog #: IC1259P
10 Citations Datasheet / COA / SDS
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IC1259P
Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nestin PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nestin.
Source
Monoclonal Mouse IgG1 Clone # 196908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Nestin antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Nestin in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 µM Retinoic Acid for 6 days (light orange filled histogram) was stained with Mouse Anti-Human Nestin PE-conjugated Monoclonal Antibody (Catalog # IC1259P) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nestin

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

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Citations for Human Nestin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. The chondrogenic differentiation potential of dental pulp stem cells
    Authors: A Longoni, L Utomo, IE van Hooijd, GK Bittermann, VC Vetter, EC Kruijt Spa, J Ross, AJ Rosenberg, D Gawlitta
    Eur Cell Mater, 2020-02-21;39(0):121-135.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. B4GALNT1 induces angiogenesis, anchorage independence growth and motility, and promotes tumorigenesis in melanoma by induction of ganglioside GM2/GD2
    Authors: H Yoshida, L Koodie, K Jacobsen, K Hanzawa, Y Miyamoto, M Yamamoto
    Sci Rep, 2020-01-27;10(1):1199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls
    Authors: AA Delbandi, M Mahmoudi, A Shervin, S Heidari, R Kolahdouz-, AH Zarnani
    BMC Womens Health, 2020-01-06;20(1):3.
    Species: Human
    Sample Types: Tissue
    Applications: Flow Cytometry
  4. Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
    Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
    Frontiers in Bioengineering and Biotechnology, 2018-11-01;0(0):2296-4185.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. The spleen of patients with myelofibrosis harbors defective mesenchymal stromal cells
    Authors: MA Avanzini, V Abbonante, P Catarsi, I Dambruoso, M Mantelli, V Poletto, E Lenta, P Guglielmel, S Croce, L Cobianchi, B Jemos, R Campanelli, E Bonetti, CA Di Buduo, S Salmoiragh, L Villani, M Massa, M Boni, R Zappatore, A Iurlo, A Rambaldi, AM Vannucchi, P Bernasconi, A Balduini, G Barosi, V Rosti
    Am. J. Hematol., 2018-02-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Phenotypic diversity of patient-derived melanoma populations in stem cell medium.
    Authors: Sztiller-Sikorska M, Hartman M, Talar B, Jakubowska J, Zalesna I, Czyz M
    Lab Invest, 2015-04-13;95(6):672-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.
    Authors: Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
    J. Invest. Dermatol., 2010-09-30;131(2):487-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. MDR1 expression identifies human melanoma stem cells.
    Authors: Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
    Biochem. Biophys. Res. Commun., 2008-02-13;368(4):930-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Isolation and characterization of renal cancer stem cells from patient-derived xenografts.
    Authors: Hasmim Meriem, Bruno Stefania, Azzi Sandy et al.
    Oncotarget

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