Human NG2/MCSP Antibody Summary
Ser1583-Ser2224
Accession # Q6UVK1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human NG2/MCSP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NG2/MCSP
NG2 (neuron/glia-type 2 antigen; also MCSP/melanoma chondroitin sulfate proteoglycan) is a 250‑500 kDa type I integral membrane proteoglycan found on synantocytes (NG2+ glia), vascular pericytes, chondroblasts, macrophages and melanoma cells. It binds multiple components of the ECM, and serves as a ligand for alpha 3 beta 1 and alpha 4 beta 1 integrins. Mature human NG2 is 2293 amino acids (aa) in length. It contains a large 2195 aa extracellular region (aa 30‑2224) and a short 79 aa cytoplasmic tail. NG2 is variably glycanated. Without proteoglycan, NG2 is expressed as a 250 kDa glycoprotein. Juxtamembrane proteolysis generates a soluble ECD that noncovalently associates with the transmembrane fragment. Over aa 1583‑2224, human NG2 is 81% aa identical to both mouse and canine NG2.
Product Datasheets
Citations for Human NG2/MCSP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Proteomic profile of melanoma cell‐derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression
Authors: Monika Pietrowska, Aneta Zebrowska, Marta Gawin, Lukasz Marczak, Priyanka Sharma, Sujan Mondal et al.
Journal of Extracellular Vesicles
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IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
Authors: Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschlager M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P
Oncogene, 2014-03-17;34(7):815-25.
Species: Human
Sample Types: Whole Tissue
Applications: IHC, IHC-Fr
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