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Human NG2/MCSP APC-conjugated Antibody

Catalog #: FAB2585A
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2585A
Detection of NG2/MCSP in A375 cells by Flow Cytometry
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NG2/MCSP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # LHM-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human melanoma cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
A375 and G361 human melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of NG2/MCSP in A375 cells by Flow Cytometry A375 cells were stained with Mouse Anti-Human NG2/MCSP APC‑conjugated Monoclonal Antibody (Catalog # FAB2585A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of NG2/MCSP antibody in G361 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP APC-conjugated Monoclonal Antibody (Catalog # FAB2585A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References
  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Human NG2/MCSP APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection
    Authors: Richards, A;Khalil, A;Friesen, M;Whitfield, TW;Lungjangwa, T;Gehrke, L;Mooney, D;Jaenisch, R;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Use of phage display biopanning as a tool to design CAR-T cells against glioma stem cells
    Authors: Potez M, Snedal S, She C et al.
    Frontiers in Oncology
  3. Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell development
    Authors: Haipeng Zhou, Ying He, Zhaoyan Wang, Qian Wang, Caiyan Hu, Xiaohua Wang et al.
    Journal of Translational Medicine
  4. Study on the Safety of Human Oligodendrocyte Precursor Cell Transplantation in Young Animals and Its Efficacy on Myelination
    Authors: Haipeng Zhou, Siliang Lu, Ke Li, Yinxiang Yang, Caiyan Hu, Zhaoyan Wang et al.
    Stem Cells and Development
  5. Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells
    Authors: G MacLeod, DA Bozek, N Rajakulend, V Monteiro, M Ahmadi, Z Steinhart, MM Kushida, H Yu, FJ Coutinho, FMG Cavalli, I Restall, X Hao, T Hart, HA Luchman, S Weiss, PB Dirks, S Angers
    Cell Rep, 2019-04-16;27(3):971-986.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Equine Mesenchymal Stromal Cells retain a pericyte-like phenotype
    Authors: CL Esteves, TA Sheldrake, L Dawson, T Menghini, E Rink, K Amilon, N Khan, B Peault, X Donadeu
    Stem Cells Dev., 2017-05-09;0(0):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Bone marrow fibroblasts parallel multiple myeloma progression in patients and mice: in vitro and in vivo studies.
    Authors: Frassanito M, Rao L, Moschetta M, Ria R, di Marzo L, De Luisi A, Racanelli V, Catacchio I, Berardi S, Basile A, Menu E, Ruggieri S, Nico B, Ribatti D, Fumarulo R, Dammacco F, Vanderkerken K, Vacca A
    2013-09-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency
    Authors: Madsen SD, Jones SH, Tucker HA et al.
    Biotechnol. Bioeng.

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