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Human NG2/MCSP Fluorescein-conjugated Antibody

Catalog #: FAB2585F
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2585F
Detection of NG2/MCSP in A375 cells by Flow Cytometry
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Citations (3)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NG2/MCSP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # LHM-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human melanoma cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
A375 and G361 human melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of NG2/MCSP in A375 cells by Flow Cytometry A375 cells were stained with Mouse Anti-Human NG2/MCSP Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2585F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of NG2/MCSP antibody in G361 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2585F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References
  1. Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
  2. Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
  3. Legg, J. et al. (2003) Development 130:6049.
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Entrez Gene IDs
1464 (Human); 121021 (Mouse); 81651 (Rat)
Alternate Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

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Citations for Human NG2/MCSP Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Destabilization of NOXA mRNA as a common resistance mechanism to targeted therapies
    Authors: J Montero, C Gstalder, DJ Kim, D Sadowicz, W Miles, M Manos, JR Cidado, J Paul Secri, AE Tron, K Flaherty, F Stephen Ho, CH Yoon, A Letai, DE Fisher, R Haq
    Nat Commun, 2019-11-14;10(1):5157.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  2. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015-01-01;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2011-12-14;318(4):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human NG2/MCSP Fluorescein-conjugated Antibody
By Christian Ostalecki on 06/07/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human

Image: Skin (melanoma); MCSP (green), Cytokeratin 14 (red), Dapi (blue)

Immunocytochemistry/Immunofluorescence Human NG2/MCSP Fluorescein-conjugated Antibody FAB2585F