Human NG2/MCSP PerCP-conjugated Antibody

Catalog #: FAB2585C Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2585C

Detection of NG2/MCSP in A375 cells by Flow Cytometry

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All NG2/MCSP Antibodies

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Human NG2/MCSP PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human NG2/MCSP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # LHM-2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human melanoma cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

A375 and G361 human melanoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NG2/MCSP in A375 cells by Flow Cytometry A375 cells were stained with Mouse Anti-Human NG2/MCSP PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2585C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry. G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP PerCP-conjugated Monoclonal Antibody (Catalog # FAB2585C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NG2/MCSP

NG2, also known as MCSP, is a chondroitin sulfate proteoglycan that has been used as a cell surface marker for melanoma (1) and glial precursor cells (2). NG2 also promotes epidermal stem cell patterned clustering (3).

References

Kupsch, J.M. et al. (1995) Melanoma Res. 5:403.
Stegmuller, J. et al. (2002) J. Neurocytol. 31:497.
Legg, J. et al. (2003) Development 130:6049.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Alternate Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8