Home / NKX2.5 / Human NKX2.5 Antibody

Human NKX2.5 Antibody

Catalog #: MAB2444
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2444
MAB2444-SP
NKX2.5 in Human Heart.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKX2.5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKX-2.5 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 259416
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry NKX2 by Immunohistochemistry (IHC-P).5 antibody in Human Heart by Immunohistochemistry (IHC-P). View Larger

NKX2.5 in Human Heart. NKX2.5 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human NKX2.5 Monoclonal Antibody (Catalog # MAB2444) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKX2.5

NKX2.5, also called NKX-2E or Csx (Cardiac specific homeobox protein), is a transcriptional activator. Its expression is essential for normal cardiovascular development. During embryonic development, NKX2.5 is also expressed in the foregut, thyroid, spleen, and stomach, while in the adult expression is predominantly restricted to the heart.

Long Name
NK2 Homeobox 5
Entrez Gene IDs
1482 (Human)
Alternate Names
cardiac-specific homeo box; cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX1; CSXFLJ97195; FLJ52202; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein Nkx-2.5; NK2 transcription factor related, locus 5 (Drosophila); NKX2.5; NKX2.5FLJ97166; NKX2-5; NKX2E; NKX2EFLJ97197; NKX4-1; tinman paralog

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Citations for Human NKX2.5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Generation of Nkx2-5/CreER Transgenic Mice for Inducible Cre Expression in Developing Hearts
    Authors: S Ranjbarvaz, S Park, NB Nguyen, WB Gilmore, P Zhao, R Ardehali
    Genesis, 2017-06-15;0(0):.
  2. Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair
    Authors: T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton
    Cell Rep, 2019-03-05;26(10):2566-2579.e10.
  3. The Biphasic Effect of Retinoic Acid Signaling Pathway on the Biased Differentiation of Atrial-like and Sinoatrial Node-like Cells from hiPSC
    Authors: Feng Liu, Dandan Long, Wenjun Huang, Wanling Peng, Huan Lan, Yafei Zhou et al.
    International Journal of Stem Cells
  4. Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency
    Authors: Wei Feng, Hannah Schriever, Shan Jiang, Abha Bais, Haodi Wu, Dennis Kostka et al.
    Communications Biology
  5. Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
    Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
    Cell Stem Cell, 2020-08-11;0(0):.
    Species: Human
    Sample Types: Organoid, Whole Cells
    Applications: IHC, ICC
  6. The involvement of protein kinase C-? in isoflurane induced preconditioning of human embryonic stem cell--derived Nkx2.5(+) cardiac progenitor cells
    Authors: IA Song, AY Oh, JH Kim, YM Choi, YT Jeon, JH Ryu, JW Hwang
    BMC Anesthesiol, 2016-02-20;16(0):13.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Essential and unexpected role of Yin Yang 1 to promote mesodermal cardiac differentiation.
    Authors: Gregoire S, Karra R, Passer D, Deutsch M, Krane M, Feistritzer R, Sturzu A, Domian I, Saga Y, Wu S
    Circ Res, 2013-01-10;112(6):900-10.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  8. Interplay of Oct4 with Sox2 and Sox17: a molecular switch from stem cell pluripotency to specifying a cardiac fate
    Authors: Sonia Stefanovic, Nesrine Abboud, Stéphanie Désilets, David Nury, Chad Cowan, Michel Pucéat
    Journal of Cell Biology
  9. Dedifferentiated fat cells convert to cardiomyocyte phenotype and repair infarcted cardiac tissue in rats.
    Authors: Jumabay M, Matsumoto T, Yokoyama S, Kano K, Kusumi Y, Masuko T, Mitsumata M, Saito S, Hirayama A, Mugishima H, Fukuda N
    J. Mol. Cell. Cardiol., 2009-08-15;47(5):565-75.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human NKX2.5 Antibody

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Human NKX2.5 Antibody
By Anonymous on 01/16/2018
Application: Flow Sample Tested: Embryonic stem cells differentiated into dopaminergic neurons Species: Human