Human NKX3.1 Antibody

Detection of Human NKX3.1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NKX3.1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

NKX3.1 in LNCaP Human Cell Line. NKX3.1 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human NKX3.1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6080) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Human NKX3.1 Antibody by Western Blot Activation of beta -catenin pathway in LNCaP cells overexpressing δ-catenin(A) Comparison of levels of δ-catenin in overexpressing LNCaP clones and other PCa cell lines. Full length protein is depicted by arrow. (B) Levels of catenin proteins in δ-catenin overexpressing LNCaP clone (designated as OE1b) are shown. Gradient loading of total protein for OE1b clone was used to illustrate an increase in expression of each protein (right panel). Quantification graphs showing protein levels normalized to GAPDH level (arbitrary units, a.u.) are at the bottom. (C) Characterization of nuclear (NE) and cytoplasmic (CE) levels of beta -catenin. Nuclear and cytoplasmic protein was isolated, beta -catenin was detected by Western blotting using specific antibody. TBP and GAPDH were used as loading control of nuclear and cytoplasmic protein respectively. Normalized level of beta -catenin in each compartment is shown at the bottom. (D and E) Characterization of levels of proteins, downstream targets of Wnt/ beta -catenin pathway (D), and androgen regulated genes AR and NKX3.1 (E) by Western blotting. Corresponding quantification is shown. Three clones (OE3a, OR1b, OE1c) overexpressing δ-catenin at various levels were examined. Total amount of 30ug of protein was used in each experiment, unless otherwise specified (in B). SDS-PAGE and Western blot conditions as in Figure 2. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29849951), licensed under a CC-BY license. Not internally tested by R&D Systems.