Human Nogo-C Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Nogo‑C by Western Blot. Western blot shows lysates of human skeletal muscle. PVDF Membrane was probed with 1 µg/mL of Human Nogo-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nogo-C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Nogo‑C in Human Brain. Nogo-C was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Human Nogo-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5766) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-C
Nogo belongs to the reticulon family of transmembrane proteins, which lack an N-terminal signal sequence and possess a 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif. They show punctate intracellular distribution within the ER that is reminescent of a reticulum. The five Nogo isoforms vary from 199 (Nogo-C) to 1192 (Nogo-A; also called reticulon-4) aa in humans. Nogo-B or -C may form complexes with Nogo-A, and all may be expressed in neurons, endothelial cells, oligodendrocytes, fibroblasts and myoblasts. Of 11 aa unique to the N-terminus of human Nogo-C, 9 and 10 aa are identical in mouse and rat Nogo-C, respectively.
Product Datasheets
Citation for Human Nogo-C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Retinal Ganglion Cell Survival and Axon Regeneration after Optic Nerve Transection is Driven by Cellular Intravitreal Sciatic Nerve Grafts
Authors: Z Ahmed, EL Suggate, A Logan, M Berry
Cells, 2020-05-27;9(6):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Nogo-C Antibody
There are currently no reviews for this product. Be the first to review Human Nogo-C Antibody and earn rewards!
Have you used Human Nogo-C Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
