Human Notch-3 Antibody

Catalog #: MAB1559
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1559
MAB1559-SP

Detection of Notch-3 in BG01V Human Stem Cells by Flow Cytometry.

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All Notch-3 Antibodies

Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Notch-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 603532

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3
Ala40-Glu467
Accession # Q9UM47

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Notch-3 in BG01V Human Stem Cells by Flow Cytometry. BGO1v human embryonic stem cells were stained with Mouse Anti-Human Notch-3 Monoclonal Antibody (Catalog # MAB1559, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry

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Detection of Notch‑3 in SUP‑T1 Human Cell Line cells by Flow Cytometry SUP‑T1 human T cell lymphoblastic lymphoma cell line was stained with Mouse Anti-Human Notch‑3 Monoclonal Antibody (Catalog # MAB1559, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-3

Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4^-CD8^- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).

Entrez Gene IDs

4854 (Human); 18131 (Mouse)

Alternate Names

CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Notch-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Therapeutic antibody targeting of Notch3 signaling prevents mural cell loss in CADASIL
Authors: Machuca-Parra AI, Bigger-Allen AA, Sanchez AV et al.
J. Exp. Med.
Active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model
Authors: DV Oliveira, KG Coupland, W Shao, S Jin, F Del Gaudio, S Wang, R Fox, JW Rutten, J Sandin, H Zetterberg, J Lundkvist, SA Lesnik Obe, U Lendahl, H Karlström
Embo Molecular Medicine, 2022-12-16;0(0):e16556.
Species: Mouse
Sample Types: Whole Blood
Applications: ELISA Capture
Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
Authors: H Kim, B Jang, YJ Kim, JC Choi
International Journal of Molecular Sciences, 2022-09-11;23(18):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
Authors: Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A
J Immunol, 2014-06-09;193(2):950-60.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, IHC