Human Notch-3 PE-conjugated Antibody

Catalog #: FAB6870P Datasheet / COA / SDS

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FAB6870P

Detection of Notch‑3 in BG01V Human Stem Cells by Flow Cytometry.

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Human Notch-3 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Notch-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Notch-3 ICD (aa 2195-2321), rhNotch-1, rhNotch-1 ICD (aa 2251-2556), rhNotch-2, rhNotch-2 ICD (aa 2063-2413), rhNotch-4, rhNotch-4 ICD (aa 1778-2003), recombinant mouse Notch-3, or recombinant rat DLL1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 603532

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Notch-3
Ala40-Glu467
Accession # Q9UM47

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Notch‑3 in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Notch-3 PE-conjugated Monoclonal Antibody (Catalog # FAB6870P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Notch‑3 in SUP‑T1 Human Cell Line by Flow Cytometry SUP‑T1 human T cell lymphoblastic lymphoma cell line was stained with Mouse Anti-Human Notch‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB6870P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Notch-3

Human Notch-3 is a member of the Notch family of type I transmembrane glycoproteins involved in early-event developmental processes. The 2321 amino acid (aa) Notch-3 precursor contains a 1603 aa extracellular region with 34 EGF-like repeats. Repeats 1-11 of human Notch-3 are within the sequence used as an immunogen, and share 94% aa identity with mouse and rat Notch-3. Repeats 11 and 12 are critical for binding the ligands Jagged and Delta. Notch-3 is expressed in vascular smooth muscle, proliferating neuroepithelium, CD4^-CD8^- thymocytes, regulatory T cells and T-ALL leukemia cells. Mutations in the first 5 EGF repeats of Notch-3 in humans can cause CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy).

Entrez Gene IDs

4854 (Human); 18131 (Mouse)

Alternate Names

CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3