Human Oct-3/4 Biotinylated Antibody

Catalog #: BAF1759 Datasheet / COA / SDS

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BAF1759

All Oct-3/4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Oct-3/4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Oct‑3/4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

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Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Oct‑3/4

Immunocytochemistry

5-15 µg/mL

Immersion fixed human and mouse embryonic stem cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oct-3/4

Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.

References

Scholer, H.R. et al. (1990) Nature 344:435.
Scholer, H.R. et al. (1989) EMBO J. 8:2543.
Rosner, M.H. et al. (1990) Nature 345:686.
Niwa, H. et al. (2000) Nat. Genet. 24:372.
Pesce, M. et al. (2001) Stem Cells 19:271.

Long Name

Octamer-binding Protein 3/4

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Alternate Names

3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1