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Human ONECUT2/OC-2 Antibody

Catalog #: AF6294
16 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6294
AF6294-SP
ONECUT2/OC-2 in HepG2 Human Cell Line.
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Product Details
Citations (16)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ONECUT2/OC-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ONECUT2/OC-2 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ONECUT2/OC-2
Gln185-Thr326
Accession # O95948
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ONECUT2/OC-2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

ONECUT2/OC-2 in HepG2 Human Cell Line. ONECUT2/OC-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human ONECUT2/OC-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

ONECUT2/OC‑2 in HeLa Human Cell Line. ONECUT2/OC‑2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human ONECUT2/OC‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6294) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ONECUT2/OC-2

ONECUT2 (one "cut" domain family member 2; also OC-2 and HNF6 beta ) is a monomeric 54 kDa (predicted) member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic neural crest plus hepatic, pancreatic, and intestinal tissue. In the adult, it is found in hepatocytes and small intestine epithelial cells. ONECUT2 is a transcriptional regulator that is known to impact the expression of Ngn3, OPN and Thbs4. This likely contributes to embryonic cell differentiation and migration. Human ONECUT2 is 504 amino acids (aa) in length. It contains two DNA binding regions, one classified as a CUT domain (aa 331‑410) and another as a homeobox domain (aa 427-481). There is one potential alternative start site at Met20. Over aa 185-326, human ONECUT2 shares 98% aa identity with mouse ONECUT2.

Long Name
One Cut Homeobox 2
Entrez Gene IDs
9480 (Human); 225631 (Mouse)
Alternate Names
one cut domain family member 2; HNF-6B; OC2; OC-2; one cut homeobox 2; ONECUT2

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Citations for Human ONECUT2/OC-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Molecular design of hypothalamus development
    Authors: Romanov R, Tretiakov E, Kastriti M et al.
    Nature
  2. Single-Cell Analysis of Human Retina Identifies Evolutionarily Conserved and Species-Specific Mechanisms Controlling Development
    Authors: Lu Y, Shiau F, Yi W et al.
    Dev. Cell
  3. A cis-regulatory module underlies retinal ganglion cell genesis and axonogenesis
    Authors: Mehta, K;Daghsni, M;Raeisossadati, R;Xu, Z;Davis, E;Naidich, A;Wang, B;Tao, S;Pi, S;Chen, W;Kostka, D;Liu, S;Gross, JM;Kuwajima, T;Aldiri, I;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. Jarid2 promotes temporal progression of retinal progenitors via repression of Foxp1
    Authors: J Zhang, JM Roberts, F Chang, J Schwakopf, ML Vetter
    Cell Reports, 2023-03-14;42(3):112237.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures
    Authors: A Sridhar, A Hoshino, CR Finkbeiner, A Chitsazan, L Dai, AK Haugan, KM Eschenbach, DL Jackson, C Trapnell, O Bermingham, I Glass, TA Reh
    Cell Rep, 2020-02-04;30(5):1644-1659.e4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits
    Authors: M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman
    Sci Rep, 2020-01-22;10(1):996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Rb1/Rbl1/Vhl loss induces mouse subretinal angiomatous proliferation and hemangioblastoma
    Authors: R Wei, X Ren, H Kong, Z Lv, Y Chen, Y Tang, Y Wang, L Xiao, S Hacibekiro, C Liang, A Nagy, R Bremner, D Chen
    JCI Insight, 2019-11-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord
    Authors: Gauhar Masgutova, Audrey Harris, Benvenuto Jacob, Lynn M. Corcoran, Frédéric Clotman
    Frontiers in Molecular Neuroscience
  9. Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord
    Authors: Audrey Harris, Gauhar Masgutova, Amandine Collin, Mathilde Toch, Maria Hidalgo-Figueroa, Benvenuto Jacob et al.
    Frontiers in Cellular Neuroscience
  10. Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice
    Authors: L Janeckova, K Kostovciko, J Svec, M Stastna, H Strnad, M Kolar, T Hudcovic, J Stancikova, J Tureckova, N Baloghova, E Sloncova, K Galuskova, H Tlaskalova, V Korinek
    Int J Mol Sci, 2019-03-29;20(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Molecular Profiling Defines Evolutionarily Conserved Transcription Factor Signatures of Major Vestibulospinal Neuron Groups
    Authors: Anders Lunde, Benjamin W. Okaty, Susan M. Dymecki, Joel C. Glover
    eNeuro
  12. Rb is required for retinal angiogenesis and lamination
    Authors: Y Zhou, R Wei, L Zhang, Y Chen, S Lu, C Liang, Y Wang, L Xiao, J Zhang, R Bremner, D Chen
    Cell Death Dis, 2018-03-06;9(3):370.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Origin and Segmental Diversity of Spinal Inhibitory Interneurons
    Authors: LB Sweeney, JB Bikoff, MI Gabitto, S Brenner-Mo, M Baek, JH Yang, EG Tabak, JS Dasen, CR Kintner, TM Jessell
    Neuron, 2018-01-04;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development
    Authors: KU Kabayiza, G Masgutova, A Harris, V Rucchin, B Jacob, F Clotman
    Front Mol Neurosci, 2017-05-26;10(0):157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells
    Authors: Silvia Velasco
    Cell Stem Cell, 2016-12-08;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation
  16. Onecut 1 and Onecut 2 are potential regulators of mouse retinal development
    Authors: Fuguo Wu, Darshan Sapkota, Renzhong Li, Xiuqian Mu
    The Journal of Comparative Neurology

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Human ONECUT2/OC-2 Antibody
By Anonymous on 09/10/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: OVCAR-3 human ovarian carcinoma cell line Species: Human
Immunocytochemistry/Immunofluorescence Human ONECUT2/OC-2 Antibody AF6294