Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 806001
View all Osteoadherin/OSAD Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human Osteoadherin/OSAD
Gln21-Gln421
Accession # Q99983

Specificity

Detects human Osteoadherin/OSAD in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Osteoadherin/OSAD Antibody

Osteoadherin/OSAD antibody in Human Mesenchymal Stem Cells by Immunocytochemistry (ICC).

Osteoadherin/OSAD in Human Mesenchymal Stem Cells.

Osteoadherin/OSAD was detected in immersion fixed human mesenchymal stem cells differentiated into osteocytes using Mouse Anti-Human Osteoadherin/OSAD Monoclonal Antibody (Catalog # MAB2884) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Osteoadherin/OSAD Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human mesenchymal stem cells differentiated into osteocytes

Reviewed Applications

Read 1 review rated 5 using MAB2884 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteoadherin/OSAD

Osteoadherin (OSAD), also known as Osteomodulin, is an extracellular matrix keratan sulfate proteoglycan that belongs to the class II subfamily of small leucine-rich proteoglycans (SLRP). LRR motifs consist of approximately 20‑30 amino acids (aa) with conserved leucine spacing, folded into a structure with one beta -sheet and one alpha -helix (1, 2). The human OSAD cDNA encodes a 421 aa precursor that contains a 20 aa signal sequence and eleven tandem leucine rich repeats (3). Human OSAD shares 80‑84% aa sequence identity with bovine, mouse, and rat OSAD. Human OSAD shares 32‑35% aa sequence identity with human class II SLRPs Fibromodulin, Keratocan, Lumican, and PRELP. Bovine, mouse, and rat OSAD are expressed as 60‑85 kDa molecules, although the amino acid sequence for each predicts a size of 46‑47 kDa. The primary difference is due to the presence of extensive N-linked glycosylation that can also vary between tissues of the same species (4, 5). Human OSAD is expressed as an even larger 110 kDa molecule in teeth (6). OSAD contains eight sulfated tyrosine residues (4, 7) and is distinguished from other class II SLRPs by the presence of an approximately 70 aa C-terminal acidic domain (3). OSAD is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon (3, 4, 8). In dental tissue, OSAD is expressed by odontoblasts and ameloblasts (5, 9‑11) and is involved in the mineralization of bone and teeth (5, 11, 12). OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3 (4, 6).

References

  1. Matsushima, N. et al. (2000) Proteins 38:210. 
  2. Kobe, B. and A.V. Kajava (2001) Curr. Opin. Struct. Biol. 11:725. 
  3. Sommarin, Y. et al. (1998) J. Biol. Chem. 273:16723. 
  4. Wendel, M. et al. (1998) J. Cell Biol. 141:839. 
  5. Hultenby, P.U. et al. (2003) Eur. J. Oral Sci. 111:128. 
  6. Lucchini, M. et al. (2004) J. Dent. Res. 83:552. 
  7. Onnerfjord, P. et al. (2004) J. Biol. Chem. 279:26
  8. Shen, Z. et al. (1999) Matrix Biol. 18:533.
  9. Buchaille, R. et al. (2000) Bone 27:265.
  10. Buchaille, R. et al. (2000) Matrix Biol. 19:421.
  11. Couble, M.L. et al. (2004) Histochem. Cell Biol. 121:47.
  12. Ramstad, V.E. et al. (2003) Calcif. Tissue Int. 72:57.

Alternate Names

OMD, OSAD, SLRR2C

Entrez Gene IDs

4958 (Human)

Gene Symbol

OMD

UniProt

Q99983

Additional Osteoadherin/OSAD Products

Product Documents for Human Osteoadherin/OSAD Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Osteoadherin/OSAD Antibody

For research use only

Customer Reviews for Human Osteoadherin/OSAD Antibody (1)

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  • Human Osteoadherin/OSAD Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 01/06/2022
    Human Osteoadherin/OSAD Antibody MAB2884

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