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Human Osteopontin (OPN) ELISA Kit - Quantikine

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Human Osteopontin/OPN ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Osteopontin (OPN) Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
0.024 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human Osteopontin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Although serum has been validated for use in this assay, it is not a recommended sample type because of proteolytic cleavage by thrombin during the clotting process. Serum values are approximately 50% of the plasma values. See references 32-34 for more details.

Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human OPN in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
EDTA plasma (n=40)94.849.2-17524.9
Heparin plasma (n=40)96.253.4-19526.7
Urine (n=21)2044122-87961951

Cell Culture Supernates:
Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 5 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human OPN.

ConditionDay 1 (ng/mL)Day 6 (ng/mL)
Unstimulated0.6751.85
Stimulated1.502.67

HepG2 human hepatocellular carcinoma cells were cultured in MEM supplemented with 5% fetal bovine serum until confluent. Cells were then stimulated with 50 ng/mL PMA for 24 hours. An aliquot of the cell culture supernate was removed, assayed for human OPN and measured 790 ng/mL.

A431 human epithelial carcinoma cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human OPN and measured 8.54 ng/mL.

Human Milk - Three human milk samples were assayed for human OPN and ranged from 35,600-248,000 ng/mL.


Product Summary

The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 2.3 4.9 9.3 2.36 4.81 9.17
Standard Deviation 0.092 0.126 0.267 0.155 0.274 0.499
CV% 4 2.6 2.9 6.6 5.7 5.4

Recovery

The recovery of Osteopontin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 86-113
EDTA Plasma (n=4) 102 92-114
Heparin Plasma (n=4) 101 95-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Osteopontin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Osteopontin/OPN ELISA Linearity

Scientific Data

N/A Human Osteopontin/OPN ELISA Standard Curve View Larger

Human Osteopontin/OPN ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Long Name:
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs:
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names:
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Osteopontin (OPN) Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

75 Citations: Showing 1 - 10
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  1. Amelioration of Photoreceptor Degeneration by Intravitreal Transplantation of Retinal Progenitor Cells in Rats
    Authors: Yang, J;Lewis, GP;Hsiang, CH;Menges, S;Luna, G;Cho, W;Turovets, N;Fisher, SK;Klassen, H;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  2. The CEBPB+ glioblastoma subcluster specifically drives the formation of M2 tumor-associated macrophages to promote malignancy growth
    Authors: Yang, Y;Jin, X;Xie, Y;Ning, C;Ai, Y;Wei, H;Xu, X;Ge, X;Yi, T;Huang, Q;Yang, X;Jiang, T;Wang, X;Piao, Y;Jin, X;
    Theranostics
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Identification of protein biomarkers associated with congenital diaphragmatic hernia in human amniotic fluid
    Authors: Bhutada, S;Tran-Lundmark, K;Kramer, B;Conner, P;Lowry, AM;Blackstone, E;Frenckner, B;Mesas-Burgos, C;Apte, SS;
    Scientific reports
    Species: Human
    Sample Types: Amniotic Fluid
  4. Macrophage-associated markers of metaflammation are linked to metabolic dysfunction in pediatric obesity
    Authors: Lischka, J;Schanzer, A;de Gier, C;Greber-Platzer, S;Zeyda, M;
    Cytokine
    Species: Human
    Sample Types: Serum
  5. Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy
    Authors: Zhao, M;Toma, K;Kinde, B;Li, L;Patel, AK;Wu, KY;Lum, MR;Tan, C;Hooper, JE;Kriegstein, AR;La Torre, A;Liao, YJ;Welsbie, DS;Hu, Y;Han, Y;Duan, X;
    Cell reports
    Species: Human
    Sample Types: Aqueous Humor
  6. Plasma Osteopontin Reflects Tissue Damage in Acute Pancreatitis
    Authors: Wirestam, L;Nyberg, PB;Dzhendov, T;Gasslander, T;Sandström, P;Sjöwall, C;Björnsson, B;
    Biomedicines
    Species: Human
    Sample Types: Plasma
  7. Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers
    Authors: Downes, KJ;Zuppa, AF;Sharova, A;Neely, MN;
    Pharmaceutics
    Species: Human
    Sample Types: Urine
  8. Matrix Metalloproteinases Contribute to the Calcification Phenotype in Pseudoxanthoma Elasticum
    Authors: Pl�mers, R;Lindenkamp, C;Osterhage, MR;Knabbe, C;Hendig, D;
    Biomolecules
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease
    Authors: Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor
    Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120.
    Species: Human
    Sample Types: Tissue Homogenates
  10. The Effect of a Comprehensive Life-Style Intervention Program of Diet and Exercise on Four Bone-Derived Proteins, FGF-23, Osteopontin, NGAL and Sclerostin, in Overweight or Obese Children and Adolescents
    Authors: SI Karampatso, G Paltoglou, SM Genitsarid, P Kassari, E Charmandar
    Nutrients, 2022-09-13;14(18):.
    Species: Human
    Sample Types: Serum
  11. Association of serum levels of osteopontin and osteoprotegerin with adverse outcomes after endovascular revascularisation in peripheral artery disease
    Authors: NPE Kadoglou, D Kapetanios, E Korakas, G Valsami, N Tentolouri, N Papanas, V Lambadiari, C Karkos
    Cardiovascular Diabetology, 2022-09-01;21(1):171.
    Species: Human
    Sample Types: Serum
  12. A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma
    Authors: Y Lu, A Yang, C Quan, Y Pan, H Zhang, Y Li, C Gao, H Lu, X Wang, P Cao, H Chen, S Lu, G Zhou
    Nature Communications, 2022-08-06;13(1):4594.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol
    Authors: MS Simon, BB Barton, C Glocker, R Musil
    PLoS ONE, 2022-07-21;17(7):e0271793.
    Species: Human
    Sample Types: Plasma
  14. Immunometabolic analysis shows a distinct cyto-metabotype in Covid-19 compared to sepsis from other causes
    Authors: FM Trovato, S Mujib, E Jerome, A Cavazza, P Morgan, J Smith, MT Depante, K O'Reilly, J Luxton, T Mare, S Napoli, MJ McPhail
    Heliyon, 2022-06-24;8(6):e09733.
    Species: Human
    Sample Types: Plasma
  15. The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis
    Authors: JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan
    Cell Death & Disease, 2022-06-06;13(6):527.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Genetics of osteopontin in patients with chronic kidney disease: The German chronic kidney disease study
    Authors: Y Cheng, Y Li, N Scherer, F Grundner-C, T Lehtimäki, BH Mishra, OT Raitakari, M Nauck, KU Eckardt, P Sekula, UT Schultheis, GCKD inves
    PloS Genetics, 2022-04-06;18(4):e1010139.
    Species: Human
    Sample Types: Serum
  17. Osteopontin-A Potential Biomarker for IgA Nephropathy: Machine Learning Application
    Authors: B Moszczuk, N Krata, W Rudnicki, B Foroncewic, D Cysewski, L P?czek, B Kaleta, K Mucha
    Biomedicines, 2022-03-22;10(4):.
    Species: Human
    Sample Types: Urine
  18. Protective Effect of Human-Neural-Crest-Derived Nasal Turbinate Stem Cells against Amyloid-beta Neurotoxicity through Inhibition of Osteopontin in a Human Cerebral Organoid Model of Alzheimer's Disease
    Authors: JY Lim, JE Lee, SA Park, SI Park, JM Yon, JA Park, SS Jeun, SJ Kim, HJ Lee, SW Kim, SH Yang
    Cells, 2022-03-18;11(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. The value of plasma hypoxia markers for predicting imaging-based hypoxia in patients with head-and-neck cancers undergoing definitive chemoradiation
    Authors: A Rühle, AL Grosu, N Wiedenmann, J Ruf, B Bieber, R Stoian, AR Thomsen, E Gkika, P Vaupel, D Baltas, WA Weber, M Mix, NH Nicolay
    Clinical and translational radiation oncology, 2022-02-21;33(0):120-127.
    Species: Human
    Sample Types: Plasma
  20. Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area
    Authors: SE Mortazavi, A Lugaajju, M Kaddumukas, MK Tijani, F Kironde, KEM Persson
    BMC microbiology, 2021-11-06;21(1):307.
    Species: Human
    Sample Types: Plasma
  21. Interleukin-33 as an early predictor of cetuximab treatment efficacy in patients with colorectal cancer
    Authors: X Zhang, K Bi, X Tu, Q Zhang, Q Cao, Y Liang, P Zeng, L Wang, T Liu, W Fang, H Diao
    Cancer Medicine, 2021-10-19;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Osteopontin Levels in Human Milk Are Related to Maternal Nutrition and Infant Health and Growth
    Authors: A Aksan, I Erdal, SS Yalcin, J Stein, G Samur
    Nutrients, 2021-07-31;13(8):.
    Species: Human
    Sample Types: Breast Milk
  23. Evaluation of a Three-Marker Panel for the Detection of Uveal Melanoma Metastases: A Single-Center Retrospective Analysis
    Authors: Z Lin, D Süsskind
    Cancers, 2021-05-18;13(10):.
    Species: Human
    Sample Types: Plasma
  24. Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinoma
    Authors: T Seki, N Yanaihara, JS Shapiro, M Saito, J Tabata, R Yokomizo, D Noguchi, T Kuroda, A Kawabata, J Suzuki, K Takahashi, H Matsuzawa, M Miyake, M Takenaka, Y Iida, S Yanagida, A Okamoto
    Scientific Reports, 2021-04-08;11(1):7689.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Lipid-injured hepatocytes release sOPN to improve macrophage migration via CD44 engagement and pFak-NF&kappaB signaling
    Authors: X Jiang, F Zhang, X Ji, F Dong, H Yu, M Xue, Y Qiu, F Yang, X Hu, Z Bao
    Cytokine, 2021-02-27;142(0):155474.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Serum
  26. Plasma osteopontin as a biomarker of Alzheimer's disease and vascular cognitive impairment
    Authors: YL Chai, JR Chong, AR Raquib, X Xu, S Hilal, N Venketasub, BY Tan, AP Kumar, G Sethi, CP Chen, MKP Lai
    Scientific Reports, 2021-02-17;11(1):4010.
    Species: Human
    Sample Types: Plasma
  27. The interaction between cancer associated fibroblasts and tumor associated macrophages via the osteopontin pathway in the tumor microenvironment of hepatocellular carcinoma
    Authors: K Tokuda, Y Morine, K Miyazaki, S Yamada, Y Saito, M Nishi, T Tokunaga, T Ikemoto, S Imura, M Shimada
    Oncotarget, 2021-02-16;12(4):333-343.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Liver stiffness measured by two-dimensional shear-wave elastography predicts hepatic vein pressure gradient at high values in liver transplant candidates with advanced liver cirrhosis
    Authors: S Frankova, M Lunova, H Gottfriedo, R Senkerikov, M Neroldova, J Kovac, E Kieslichov, V Lanska, P Urbanek, J Spicak, M Jirsa, J Sperl
    PLoS ONE, 2021-01-07;16(1):e0244934.
    Species: Human
    Sample Types: Plasma
  29. ASP5094, a humanized monoclonal antibody against integrin alpha-9, did not show efficacy in patients with rheumatoid arthritis refractory to methotrexate: results from a phase 2a, randomized, double-blind, placebo-controlled trial
    Authors: T Takeuchi, Y Tanaka, J Erdman, Y Kaneko, M Saito, C Higashitan, R Smulders, C Lademacher
    Arthritis Res Ther, 2020-10-21;22(1):252.
    Species: Human
    Sample Types: Plasma
  30. Prognostic significance of serum osteopontin levels in small cell lung cancer
    Authors: C Xu, Q Yuan, W Wang, C Chi, Q Zhang, L Li, R Yang, Y Wang
    BMC Pulm Med, 2020-09-01;20(1):235.
    Species: Human
    Sample Types: Serum
  31. Liver osteopontin is required to prevent the progression of age-related nonalcoholic fatty liver disease
    Authors: B Gómez-Sant, D Saenz de U, M Nuñez-Garc, F Gonzalez-R, X Buque, I Aurrekoetx, V Gutiérrez, MJ Gonzalez-R, C García-Mon, Á González-R, L Mosteiro, G Errazti, P Mifsut, S Gaztambide, L Castaño, C Martin, R Nogueiras, ML Martinez-C, WK Syn, P Aspichueta
    Aging Cell, 2020-07-07;0(0):e13183.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus
    Authors: C Wang, M He, J Peng, S Li, M Long, W Chen, D Liu, G Yang, L Zhang
    Cytokine, 2019-09-09;125(0):154837.
    Species: Human
    Sample Types: Plasma
  33. Blood serum proteins as biomarkers for prediction of survival, locoregional control and distant metastasis rate in radiotherapy and radio-chemotherapy for non-small cell lung cancer
    Authors: R Suwinski, M Giglok, K Galwas-Kli, A Idasiak, B Jochymek, R Deja, B Maslyk, J Mrochem-Kw, D Butkiewicz
    BMC Cancer, 2019-05-08;19(1):427.
    Species: Human
    Sample Types: Plasma
  34. Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization
    Authors: SH Choi, AR Kim, JK Nam, JM Kim, JY Kim, HR Seo, HJ Lee, J Cho, YJ Lee
    Nat Commun, 2018-11-30;9(1):5108.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018-11-08;7(11):.
    Species: Human
    Sample Types: Plasma
  36. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion
    Authors: JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu
    J. Clin. Invest., 2018-11-05;0(0):.
    Species: Human
    Sample Types: Serum
  37. Assessment of Cross-correlations Between Selected Macromolecules in Urine of Children with Idiopathic Hypercalciuria
    Authors: K Jobs, A Jung, S Lewicki, P Murawski, L P?czek, R Zdanowski
    Urol J, 2018-09-26;0(0):.
    Species: Human
    Sample Types: Urine
  38. Upregulation of osteopontin expression via the interaction of macrophages and fibroblasts under IL-1b stimulation
    Authors: T Shimodaira, K Matsuda, T Uchibori, M Sugano, T Uehara, T Honda
    Cytokine, 2018-04-25;110(0):63-69.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Methylglyoxal-bis-guanylhydrazone inhibits osteopontin expression and differentiation in cultured human monocytes
    Authors: X Jin, H Xu, MS McGrath
    PLoS ONE, 2018-03-14;13(3):e0192680.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Association of plasma osteopontin with diabetic retinopathy in Asians with type 2 diabetes
    Authors: X Zhang, WK Chee, S Liu, S Tavinthara, CF Sum, SC Lim, N Kumari
    Mol. Vis., 2018-02-15;24(0):165-173.
    Species: Human
    Sample Types: Plasma
  41. Effects of oral paricalcitol therapy on arterial stiffness and osteopontin in hypertensive patients with chronic kidney disease and secondary hyperparathyroidism
    Authors: M Giakoumis, C Tsioufis, K Dimitriadi, M Sonikian, A Kasiakogia, E Andrikou, T Kalos, D Konstantin, K Filis, D Petras, D Tousoulis
    Hellenic J Cardiol, 2018-01-04;0(0):.
    Species: Human
    Sample Types: Whole Blood
  42. Osteopontin is Critical for Hyperactive mTOR-Induced Tumorigenesis in Oral Squamous Cell Carcinoma
    Authors: N Gan, S Zou, W Hang, D Yang, X Zhang, Y Yin
    J Cancer, 2017-05-12;8(8):1362-1370.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis
    PLoS ONE, 2016-12-09;11(12):e0167435.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
    JCI Insight, 2016-12-08;1(20):e90954.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016-11-15;90(0):88-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Targeting of the leukemia microenvironment by c(RGDfV) overcomes the resistance to chemotherapy in acute myeloid leukemia in biomimetic polystyrene scaffolds
    Oncol Lett, 2016-08-24;12(5):3278-3284.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Immunomodulation Induced by Stem Cell Mobilization and Harvesting in Healthy Donors: Increased Systemic Osteopontin Levels after Treatment with Granulocyte Colony-Stimulating Factor
    Int J Mol Sci, 2016-07-19;17(7):.
    Species: Human
    Sample Types: Plasma
  48. Osteopontin is a multi-faceted pro-tumorigenic driver for central nervous system lymphoma
    Oncotarget, 2016-05-31;7(22):32156-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Biomarkers of inflammation and axonal degeneration/damage in patients with newly diagnosed multiple sclerosis: contributions of the soluble CD163 CSF/serum ratio to a biomarker panel.
    Authors: Stilund M, Gjelstrup M, Petersen T, Moller H, Rasmussen P, Christensen T
    PLoS ONE, 2015-04-10;10(4):e0119681.
    Species: Human
    Sample Types: Serum
  50. Prediction of cardiovascular events in statin-treated stable coronary patients of the treating to new targets randomized controlled trial by lipid and non-lipid biomarkers.
    Authors: Arsenault B, Barter P, DeMicco D, Bao W, Preston G, LaRosa J, Grundy S, Deedwania P, Greten H, Wenger N, Shepherd J, Waters D, Kastelein J
    PLoS ONE, 2014-12-22;9(12):e114519.
    Species: Human
    Sample Types: Plasma
  51. Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study.
    Authors: Aderhold C, Grobschmidt G, Sauter A, Faber A, Hormann K, Schultz J
    Oncol Lett, 2014-07-04;8(3):1112-1118.
    Species: Human
    Sample Types: Serum
  52. Prognostic value of secreted phosphoprotein-1 in pleural effusion associated with non-small cell lung cancer.
    Authors: Zhang, He, Liu, Hong-bin, Yuan, Dong-mei, Wang, Zhao-fen, Wang, Yun-fen, Song, Yong
    BMC Cancer, 2014-04-23;14(0):280.
    Species: Human
    Sample Types: Pleural Fluid
  53. Osteopontin is a prognostic biomarker in non-small cell lung cancer.
    Authors: Rud A, Boye K, Oijordsbakken M, Lund-Iversen M, Halvorsen A, Solberg S, Berge G, Helland A, Brustugun O, Maelandsmo G
    BMC Cancer, 2013-11-11;13(0):540.
    Species: Human
    Sample Types: Serum
  54. Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.
    Authors: Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L
    J Biol Chem, 2012-11-30;288(5):3097-111.
    Species: Human
    Sample Types: Tissue Homogenates
  55. A multi-analyte assay for the non-invasive detection of bladder cancer.
    Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
    PLoS ONE, 2012-10-19;7(10):e47469.
    Species: Human
    Sample Types: Urine
  56. Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma.
    Br. J. Cancer, 2012-08-23;107(7):1131-7.
    Species: Human
    Sample Types: Plasma
  57. Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients.
    Authors: Liersch R, Gerss J, Schliemann C, Bayer M, Schwoppe C, Biermann C, Appelmann I, Kessler T, Lowenberg B, Buchner T, Hiddemann W, Muller-Tidow C, Berdel WE, Mesters R
    Blood, 2012-04-17;119(22):5215-20.
    Species: Human
    Sample Types: Plasma
  58. An in vivo platform for tumor biomarker assessment.
    Authors: Servais EL, Suzuki K, Colovos C
    PLoS ONE, 2011-10-26;6(10):e26722.
    Species: Human
    Sample Types: Plasma
  59. Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma.
    Authors: Zekri A, Bahnassy A, Alam El-Din H, Morsy H, Shaarawy S, Moharram N, Daoud S
    Oncol Rep, 2011-06-21;26(4):825-31.
    Species: Human
    Sample Types: Serum
  60. Plasma markers for identifying patients with metastatic melanoma.
    Authors: Kluger HM, Hoyt K, Bacchiocchi A, Mayer T, Kirsch J, Kluger Y, Sznol M, Ariyan S, Molinaro A, Halaban R
    Clin. Cancer Res., 2011-04-12;17(8):2417-25.
    Species: Human
    Sample Types: Plasma
  61. A multi-analyte serum test for the detection of non-small cell lung cancer.
    Authors: Farlow EC, Vercillo MS, Coon JS, Basu S, Kim AW, Faber LP, Warren WH, Bonomi P, Liptay MJ, Borgia JA
    Br. J. Cancer, 2010-09-21;103(8):1221-8.
    Species: Human
    Sample Types: Serum
  62. Changes in osteopontin and in biomarkers of bone turnover during human endotoxemia.
    Authors: Grimm G, Vila G, Bieglmayer C
    Bone, 2010-04-24;47(2):388-91.
    Species: Human
    Sample Types: Plasma
  63. Osteopontin as a novel prognostic marker in stable ischaemic heart disease: a 3-year follow-up study.
    Authors: Georgiadou P, Iliodromitis EK, Kolokathis F, Varounis C, Gizas V, Mavroidis M, Capetanaki Y, Boudoulas H, Kremastinos DT
    Eur. J. Clin. Invest., 2010-04-01;0(0):.
    Species: Human
    Sample Types: Plasma
  64. Circulating levels of osteopontin are closely related to glomerular filtration rate and cardiovascular risk markers in patients with chronic kidney disease.
    Authors: Lorenzen J, Kramer R, Kliem V, Bode-Boeger SM, Veldink H, Haller H, Fliser D, Kielstein JT
    Eur. J. Clin. Invest., 2010-04-01;40(4):294-300.
    Species: Human
    Sample Types: Plasma
  65. Osteopontin is expressed and functional in human eosinophils.
    Authors: Puxeddu I, Berkman N, Ribatti D
    Allergy, 2009-10-05;65(2):168-74.
    Species: Human
    Sample Types: BALF
  66. Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome.
    Authors: Campeau PM, Rafei M, Boivin MN, Sun Y, Grabowski GA, Galipeau J
    Blood, 2009-07-08;114(15):3181-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Activation of the osteopontin/matrix metalloproteinase-9 pathway correlates with prostate cancer progression.
    Authors: Castellano G, Malaponte G, Mazzarino MC, Figini M, Marchese F, Gangemi P, Travali S, Stivala F, Canevari S, Libra M
    Clin. Cancer Res., 2008-11-15;14(22):7470-80.
    Species: Human
    Sample Types: Plasma
  68. Abrogation of T cell quiescence characterizes patients at high risk for multiple sclerosis after the initial neurological event.
    Authors: Corvol JC, Pelletier D, Henry RG, Caillier SJ, Wang J, Pappas D, Casazza S, Okuda DT, Hauser SL, Oksenberg JR, Baranzini SE
    Proc. Natl. Acad. Sci. U.S.A., 2008-08-08;105(33):11839-44.
    Species: Human
    Sample Types: Plasma
  69. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
    Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
    Arch. Immunol. Ther. Exp. (Warsz.), 2008-05-30;56(3):207-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Intensive lipid-lowering therapy ameliorates novel calcification markers and GSM score in patients with carotid stenosis.
    Authors: Kadoglou NP, Gerasimidis T, Moumtzouoglou A, Kapelouzou A, Sailer N, Fotiadis G, Vitta I, Katinios A, Kougias P, Bandios S, Voliotis K, Karayannacos PE, Liapis CD
    Eur J Vasc Endovasc Surg, 2008-04-18;35(6):661-8.
    Species: Human
    Sample Types: Plasma
  71. Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide.
    Authors: Dower K, Ellis DK, Saraf K, Jelinsky SA, Lin LL
    J. Immunol., 2008-03-01;180(5):3520-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
    Authors: Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N
    Blood, 2007-09-11;110(13):4464-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin.
    Authors: Shao J, Washington MK, Saxena R, Sheng H
    Carcinogenesis, 2007-08-11;28(12):2476-83.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma.
    Authors: Meinhold-Heerlein I, Bauerschlag D, Zhou Y, Sapinoso LM, Ching K, Frierson H, Brautigam K, Sehouli J, Stickeler E, Konsgen D, Hilpert F, von Kaisenberg CS, Pfisterer J, Bauknecht T, Jonat W, Arnold N, Hampton GM
    Clin. Cancer Res., 2007-01-15;13(2):458-66.
    Species: Human
    Sample Types: Serum
  75. Osteopontin expression and serum levels in metastatic uveal melanoma: a pilot study.
    Authors: Pe&amp;apos;er J
    Invest. Ophthalmol. Vis. Sci., 2006-03-01;47(3):802-6.
    Species: Human
    Sample Types: Serum

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Human Osteopontin (OPN) Quantikine ELISA Kit
By Alicia Furlan on 05/15/2024
Sample Tested: EDTA Plasma
Human Osteopontin (OPN) Quantikine ELISA Kit
By Anonymous on 11/03/2023
Sample Tested: Plasma,Urine

Measured plasma at 25-fold dilution and urine at 200-fold dilution. We were able to obtain values in range for both biofluids.

Human Osteopontin (OPN) Quantikine ELISA Kit DOST00
Human Osteopontin (OPN) Quantikine ELISA Kit
By Anonymous on 02/18/2021
Sample Tested: Serum
Human Osteopontin (OPN) Quantikine ELISA Kit DOST00
Human Osteopontin (OPN) Quantikine ELISA Kit
By Konrad Gabrusiewicz on 12/01/2017
Sample Tested: Human cell conditioned medium