Human PBEF/Visfatin Antibody

Catalog #: MAB4335 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB4335-100
MAB4335-SP

Human PBEF/Visfatin ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PBEF/Visfatin Antibody Summary

Species Reactivity

Human

Specificity

Detects human PBEF/Visfatin in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 882115R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant Human PBEF/Visfatin
Pro27-His491
Accession # P43490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human PBEF/Visfatin DuoSet ELISA Kit (Catalog # DY4335-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human PBEF/Visfatin ELISA Standard Curve. Recombinant Human PBEF/Visfatin protein was serially diluted 2-fold and captured by Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB4335) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human PBEF/Visfatin Monoclonal Antibody (Catalog # MAB43351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Alternate Names

EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin