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Human PDGF Protein, CF

Catalog #: 120-HD
19 Citations Datasheet / COA / SDS
Newer Version Available: 222-AB
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Discontinued Product

120-HD has been discontinued and is replaced by 222-AB.

Product Details
Citations (19)
FAQs
Reviews
Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Human PDGF Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
Source
Human platelet-derived PDGF protein
The human platelets used for the isolation of this product was certified by the supplier to be HIV 1/2 Ab, HCV Ab, HTLV 1/2, HBc Ab and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.
Structure / Form
Mostly disulfide-linked heterodimer
SDS-PAGE
Multiple bands between 28-31 kDa, non-reducing conditions

Product Datasheets

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120-HD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

120-HD

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute 1 µg vials at 10 µg/mL in sterile 4 mM HCl. Reconstitute 5 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems hPDGF consists predominantly of hPDGF-AB heterodimers.

Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Long Name
Platelet-derived Growth Factor
Entrez Gene IDs
5154 (Human)
Alternate Names
PDGF; PDGF1; PDGF-1; PDGFA; PDGF-A

Citations for Human PDGF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
    Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
    Sci Rep, 2020-07-31;10(1):12912.
    Species: Snake
    Sample Types: Protein
    Applications: Bioassay
  2. The Secretome Analysis of Activated Human Renal Fibroblasts Revealed Beneficial Effect of the Modulation of the Secreted Peptidyl-Prolyl Cis-Trans Isomerase A in Kidney Fibrosis
    Authors: GH Dihazi, M Eltoweissy, O Jahn, B Tampe, M Zeisberg, HS Wülfrath, GA Müller, H Dihazi
    Cells, 2020-07-18;9(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Trefoil Factor 3 (TFF3) Is Involved in Cell Migration for Skeletal Repair
    Authors: K Krüger, S Schmid, F Paulsen, A Ignatius, P Klinger, T Hotfiel, B Swoboda, K Gelse
    Int J Mol Sci, 2019-09-01;20(17):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. CAMKII as a therapeutic target for growth factor-induced retinal and choroidal neovascularisation
    Authors: S Ashraf, S Bell, C O'Leary, P Canning, I Micu, JA Fernandez, M O'Hare, P Barabas, H McCauley, DP Brazil, AW Stitt, JG McGeown, TM Curtis
    JCI Insight, 2019-03-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Circulating Apoptotic Signals During Acute and Chronic Exposure to High Altitude in Kyrgyz Population
    Authors: D Kosanovic, SM Platzek, A Petrovic, A Sydykov, A Maripov, A Mamazhakyp, M Sartmyrzae, K Muratali U, M Cholponbae, A Toktosunov, N Omurzakova, M Duishobaev, C Vroom, O Pak, N Weissmann, HA Ghofrani, A Sarybaev, RT Schermuly
    Front Physiol, 2019-02-05;10(0):54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. IL-33 Initiates Vascular Remodelling in Hypoxic Pulmonary Hypertension by up-Regulating HIF-1? and VEGF Expression in Vascular Endothelial Cells
    Authors: J Liu, W Wang, L Wang, S Chen, B Tian, K Huang, CJ Corrigan, S Ying, W Wang, C Wang
    EBioMedicine, 2018-06-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Endothelial nitric oxide synthase overexpressing human early outgrowth cells inhibit coronary artery smooth muscle cell migration through paracrine functions
    Authors: S Guber, T Ebrahimian, M Heidari, N Eliopoulos, S Lehoux
    Sci Rep, 2018-01-17;8(1):877.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Umbilical cord mesenchymal stem cell transplantation in the treatment of multiple sclerosis
    Authors: M Meng, Y Liu, W Wang, C Wei, F Liu, Z Du, Y Xie, W Tang, Z Hou, Q Li
    Am J Transl Res, 2018-01-15;10(1):212-223.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  9. Characterization of an iron oxide nanoparticle labelling and MRI-based protocol for inducing human mesenchymal stem cells into neural-like cells
    Authors: CW Lu, JK Hsiao, HM Liu, CH Wu
    Sci Rep, 2017-06-15;7(1):3587.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium.
    Authors: Lynne A Murray, Rebecca Dunmore, Ana Camelo, Carla A Da Silva, Malin J Gustavsso, David M Habiel, Tillie L Hackett, Cory M Hogaboam, Matthew A Sleeman, Darryl A Knight
    Respiratory Research, 2017-05-03;0(0):1465-993X.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration
    J Exp Clin Cancer Res, 2016-07-29;35(1):122.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Transcription factor TBX4 regulates myofibroblast accumulation and lung fibrosis
    J Clin Invest, 2016-07-11;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. A Small Molecule Inhibitor of PDK1/PLC?1 Interaction Blocks Breast and Melanoma Cancer Cell Invasion
    Authors: C Raimondi, V Calleja, R Ferro, A Fantin, AM Riley, BV Potter, CH Brennan, T Maffucci, B Larijani, M Falasca
    Sci Rep, 2016-05-20;6(0):26142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Smooth Muscle Enriched Long Non-Coding RNA (SMILR) Regulates Cell Proliferation
    Authors: MD Ballantyne, K Pinel, R Dakin, AT Vesey, L Diver, R Mackenzie, R Garcia, P Welsh, N Sattar, G Hamilton, N Joshi, MR Dweck, JM Miano, MW McBride, DE Newby, RA McDonald, AH Baker
    Circulation, 2016-04-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Experimentally-derived fibroblast gene signatures identify molecular pathways associated with distinct subsets of systemic sclerosis patients in three independent cohorts.
    Authors: Johnson M, Mahoney J, Taroni J, Sargent J, Marmarelis E, Wu M, Varga J, Hinchcliff M, Whitfield M
    PLoS ONE, 2015-01-21;10(1):e0114017.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Clinical-grade multipotent adult progenitor cells durably control pathogenic T cell responses in human models of transplantation and autoimmunity.
    Authors: Reading J, Yang J, Sabbah S, Skowera A, Knight R, Pinxteren J, Vaes B, Allsopp T, Ting A, Busch S, Raber A, Deans R, Tree T
    J Immunol, 2013-04-01;190(9):4542-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009-01-20;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
    Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
    J Clin Invest, 1998-02-15;101(4):890-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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