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Human Pepsinogen A5/PGA5 Antibody

Catalog #: AF8457 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8457-100
AF8457-SP
Detection of Human Pepsinogen A5/PGA5 by Western Blot.
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Human Pepsinogen A5/PGA5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pepsinogen A5/PGA5 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A5/PGA5
Met1-Ala388
Accession # P0DJD7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Pepsinogen A5/PGA5 antibody by Western Blot. View Larger

Detection of Human Pepsinogen A5/PGA5 by Western Blot. Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Pepsinogen A5/PGA5 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Pepsinogen A5/PGA5 by Simple WesternTM. Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pepsinogen A5/PGA5

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA-3, PGA-4, and PGA-5 are isozymogens of human Pepsinogen A, which differ in amino acid sequence by 2-4 residues (2). This recombinant human Pepsinogen A corresponds to PGA-4. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

References
  1. Athauda, S.B. et al. (1989) J. Biochem. 106:920.
  2. Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.
Entrez Gene IDs
5222 (Human)
Alternate Names
EC 3.4.23; EC 3.4.23.1; pepsin A; pepsinogen 5, group I (pepsinogen A); Pepsinogen A5; PGA3; PGA4; PGA5

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