Human Phospho-ER alpha /NR3A1 (S118) Antibody

Catalog #: AF5954 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5954
AF5954-SP

Detection of Human Phospho‑ER alpha /NR3A1 (S118) by Western Blot.

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Human Phospho-ER alpha /NR3A1 (S118) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-ER alpha /NR3A1 (S118) in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing the human ER alpha /NR3A1 S118 site
Accession # P03372

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho‑ER alpha /NR3A1 (S118) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 nM estradiol for 60 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-ERa/NR3A1 (S118) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5954) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-ERa/NR3A1 (S118) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ER alpha/NR3A1

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65‑70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta -estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple co-activators. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Phosphorylation at Ser118 regulates ER alpha 's role in transcription and cell growth.

Long Name

Estrogen Receptor alpha

Entrez Gene IDs

2099 (Human)

Alternate Names

ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1