Home / MSPR/Ron / Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody

Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody

Catalog #: AF1947
13 Citations Datasheet / COA / SDS
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AF1947
AF1947-SP
Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot.
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Citations (13)
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Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody Summary

Species Reactivity
Human
Specificity
The antibody detects endogenous human MSP R/Ron phosphorylated at Y1238/Y1239 using Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
Phosphopeptide containing human MSP R/Ron Y1238/Y1239 sites
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) antibody by Western Blot. View Larger

Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot. Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) antibody by Western Blot. View Larger

Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western blot. Human Phospho-MSP R/Ron (Y1238/Y1239) was immunoprecipitated from 1 mg of lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947).

Western Blot Detection of Human Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody by Western Blot View Larger

Detection of Human Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody by Western Blot The expression and activity of Ron receptor isoforms in primary tumors and high-grade serous ovarian (HG-SOC) PDXsA. Upper panel displays western blot analysis of tumor lysates from patients assayed for phosphorylated (active) sfRon and Ron. Lower panel shows western blot analysis of total levels of Ron isoforms. The higher molecular weight sfRon bands (sfRon-HMW), which are putative, posttranslationally modified sfRon forms were also noted. The blots were stripped and re-probed for beta -actin. Lanes represent: healthy ovary (1, 2, 3); ovarian adenocarcinoma (4); carcinosarcoma (5); endometrioid adenocarcinoma (6) and HG-SOC (7). B. Upper panel displays western blot analysis of tumor lysates from HG-SOC PDXs assayed for phosphorylated Ron isoforms. Lower panel shows western blot analysis of total levels of Ron isoforms. The blots were stripped and re-probed for beta -actin or GAPDH. C. The expression of Ron isoforms was assessed by WES capillary electrophoresis-based protein assay in OVCAR3-sfRon cell line engineered to express sfRon vs. parental OVACR3 cells and compared with sfRon positive or negative HG-SOC PDXs. GAPDH was used as loading control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27551332), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), also known as Ron, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met and chicken Sea. Each family member is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha  chain and a membrane-spanning beta  chain with intrinsic tyrosine kinase activity. MSP R/Ron is overexpressed in a number of primary breast carcinomas and non-small cell lung carcinomas, suggesting a role for this RTK in mammary and lung tumor progression. Phosphorylation of MSP R/Ron at Y1238/Y1239 in the activation loop of the kinase domain is responsible for kinase activity.

Long Name
Macrophage Stimulating Protein Receptor
Entrez Gene IDs
4486 (Human); 19882 (Mouse)
Alternate Names
CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4

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Citations for Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. The Receptor Tyrosine Kinase RON and Its Isoforms as Therapeutic Targets in Ewing Sarcoma
    Authors: P Berning, C Hennemann, C Tulotta, C Schaefer, B Lechtape, M Hotfilder, Y El Gourari, H Jürgens, E Snaar-Jaga, G Hempel, U Dirksen, J Potratz
    Cancers (Basel), 2020-04-07;12(4):.
  2. Effects of heterologous kinase domains on growth factor receptor specificity
    Authors: Hayashi, SY;Craddock, BP;Miller, WT;
    Cellular signalling
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Crizotinib Has Preclinical Efficacy in Philadelphia-Negative Myeloproliferative Neoplasms
    Authors: Lindsay M. Gurska, Rachel Okabe, Alexandra Schurer, Meng Maxine Tong, Mark Soto, Daniel Choi et al.
    Clinical Cancer Research
  4. Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression
    Authors: DM Beauvais, SE Nelson, KM Adams, NA Stueven, O Jung, AC Rapraeger
    The Journal of Biological Chemistry, 2022-05-13;0(0):102029.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. EGFR transactivates RON to drive oncogenic crosstalk
    Authors: Carolina Franco Nitta, Ellen W Green, Elton D Jhamba, Justine M Keth, Iraís Ortiz-Caraveo, Rachel M Grattan et al.
    eLife
  6. Recepteur d'origine nantais contributes to the development of endometriosis via promoting epithelial-mesenchymal transition of a endometrial epithelial cells
    Authors: Q Yu, J Wang, T Li, X Guo, S Ding, X Che, L Zhu, Y Peng, X Xu, G Zou, X Zhang
    Journal of Cellular and Molecular Medicine, 2021-01-06;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. RON signalling promotes therapeutic resistance in ESR1 mutant breast cancer
    Authors: D Dustin, G Gu, AR Beyer, SK Herzog, DG Edwards, H Lin, TL Gonzalez, SL Grimm, C Coarfa, DW Chan, BJ Kim, JP De La O, MJ Ellis, D Liu, S Li, AL Welm, SAW Fuqua
    British Journal of Cancer, 2020-12-01;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Analysis of the Effect of Increased &alpha2,3-Sialylation on RTK Activation in MKN45 Gastric Cancer Spheroids Treated with Crizotinib
    Authors: M Balmaña, F Diniz, T Feijão, CC Barrias, S Mereiter, CA Reis
    Int J Mol Sci, 2020-01-22;21(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Broad-spectrum receptor tyrosine kinase inhibitors overcome de novo and acquired modes of resistance to EGFR-targeted therapies in colorectal cancer
    Authors: R Graves-Dea, G Bogatcheva, S Rehman, Y Lu, JN Higginboth, B Singh
    Oncotarget, 2019-02-12;10(13):1320-1333.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. HGFL-mediated RON signaling supports breast cancer stem cell phenotypes via activation of non-canonical beta -catenin signaling
    Authors: Sasha J. Ruiz-Torres, Nancy M. Benight, Rebekah A. Karns, Elyse E. Lower, Jun-Lin Guan, Susan E. Waltz
    Oncotarget
  11. Characterization of RON protein isoforms in pancreatic cancer: implications for biology and therapeutics
    Authors: Jeffery Chakedis, Randall French, Michele Babicky, Dawn Jaquish, Evangeline Mose, Peter Cheng et al.
    Oncotarget
  12. Genetic suppression of inflammation blocks the tumor-promoting effects of TGF-beta in gastric tissue.
    Authors: Ota M, Horiguchi M, Fang V, Shibahara K, Kadota K, Loomis C, Cammer M, Rifkin D
    Cancer Res, 2014-03-03;74(9):2642-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. The Ron receptor tyrosine kinase positively regulates angiogenic chemokine production in prostate cancer cells.
    Authors: Thobe MN, Gurusamy D, Pathrose P, Waltz SE
    Oncogene, 2009-10-19;29(2):214-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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