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Human Phospho-p53 (S20) Antibody

Catalog #: AF2286
2 Citations Datasheet / COA / SDS

Discontinued Product

AF2286 has been discontinued.
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Detection of Human Phospho-p53 (S20) by Western Blot.
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Citations (2)
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Human Phospho-p53 (S20) Antibody Summary

Species Reactivity
Human
Specificity
Detects human p53 when phosphorylated at S20 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human p53 S20 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-p53 (S20) antibody by Western Blot. View Larger

Detection of Human Phospho-p53 (S20) by Western Blot. Western blot shows p53 immunoprecipitated from lysates of MCF-7 human breast cancer cell line using Human/Mouse/Rat p53 Agarose-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # GAF1355). MCF-7 cell line was untreated (-) or exposed (+) to 10 Gy ionizing radiation (IR) for 3 hours. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p53 (S20) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2286) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p53 (S20) was detected at approximately 55 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U ?-phosphatase (?-PPase) for 1 hour. For additional reference the membrane was stripped and reprobed with 1:5000 dilution of Human/Mouse/Rat p53 HRP-conjugated Antigen Affinity-purified Polyclonal Antibody (lower panel, Catalog # HAF1355) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p53

The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The Chk1 or Chk2 kinases phosphorylate p53 at serine 20 to enforce cell cycle arrest. Serine 20 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.

Entrez Gene IDs
7157 (Human); 22059 (Mouse); 24842 (Rat)
Alternate Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53

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Citations for Human Phospho-p53 (S20) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. DNA damage signaling in response to 5-fluorouracil in three colorectal cancer cell lines with different mismatch repair and TP53 status.
    Authors: Adamsen BL, Kravik KL, De Angelis PM
    Int. J. Oncol., 2011-06-14;39(3):673-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
    Authors: Rozan LM, McDonald ER, Navaraj A, Matthew EM, Dicker DT, El-Deiry WS
    J. Biol. Chem., 2006-11-22;282(5):3273-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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