Home / VEGFR1/Flt-1 / Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

Catalog #: AF4170
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4170
AF4170-SP
Detection of Human Phospho-VEGFR1/Flt‑1 (Y1213) by Western Blot.
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Citations (14)
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Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR1/Flt-1 when phosphorylated at Y1213.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human VEGFR1/Flt-1 Y1213 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Phospho-VEGFR1/Flt‑1 (Y1213) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-VEGFR1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

VEGFR1, also known as Flt-1, is a receptor tyrosine kinase that contains seven Ig-like repeats in its extracellular domain, a single transmembrane region, and a kinase domain in the intracellular region. Ligand binding induces autophosphorylation on multiple intracellular tyrosine residues. Y1213 is a major binding site for PI3K, Nck, and SHP-2.

Long Name
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs
2321 (Human); 14254 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Hypoxia-autophagy axis induces VEGFA by peritoneal mesothelial cells to promote gastric cancer peritoneal metastasis through an integrin &alpha5-fibronectin pathway
    Authors: X Wang, X Che, Y Yu, Y Cheng, M Bai, Z Yang, Q Guo, X Xie, D Li, M Guo, K Hou, W Guo, X Qu, L Cao
    J Exp Clin Cancer Res, 2020-10-20;39(1):221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. lncRNA PVT1 Promotes Tumorigenesis of Colorectal Cancer by Stabilizing miR-16-5p and Interacting with the VEGFA/VEGFR1/AKT Axis
    Authors: Hailu Wu, Ming Wei, Xinglu Jiang, Jiacheng Tan, Wei Xu, Xiaobo Fan et al.
    Molecular Therapy - Nucleic Acids
  3. Identification of Cross Talk between FoxM1 and RASSF1A as a Therapeutic Target of Colon Cancer
    Authors: Thomas G. Blanchard, Steven J. Czinn, Vivekjyoti Banerjee, Neha Sharda, Andrea C. Bafford, Fahad Mubariz et al.
    Cancers (Basel)
  4. Treatment with placental growth factor attenuates myocardial ischemia/reperfusion injury
    Authors: Y Zhang, C Cao, J Xin, P Lv, D Chen, S Li, H Yang, C Chen, B Liu, Q Li
    PLoS ONE, 2018-09-13;13(9):e0202772.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation
    Authors: TG Blanchard, R Lapidus, V Banerjee, AC Bafford, SJ Czinn, H Ahmed, A Banerjee
    Cell. Physiol. Biochem., 2018-07-25;48(3):1259-1273.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells
    Authors: MG Atzori, L Tentori, F Ruffini, C Ceci, L Lisi, E Bonanno, M Scimeca, E Eskilsson, T Daubon, H Miletic, L Ricci Viti, R Pallini, P Navarra, R Bjerkvig, S D'Atri, PM Lacal, G Graziani
    J. Exp. Clin. Cancer Res., 2017-08-10;36(1):106.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Intracrine VEGF signalling mediates colorectal cancer cell migration and invasion
    Authors: R Bhattachar, F Fan, R Wang, X Ye, L Xia, D Boulbes, LM Ellis
    Br. J. Cancer, 2017-07-25;117(6):848-855.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  8. BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
    Authors: Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R
    Clin Cancer Res, 2015-01-23;21(7):1652-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1alpha-VEGF pathway inhibition.
    Authors: Huang H, He J, Johnson D, Wei Y, Liu Y, Wang S, Lutty G, Duh E, Semba R
    Diabetes, 2014-09-03;64(1):200-12.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
    Authors: Meda C, Molla F, De Pizzol M
    J. Immunol., 2012-03-21;188(8):4081-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells.
    Authors: Attar-Schneider O, Drucker L, Zismanov V
    Lab. Invest., 2011-11-14;92(2):178-90.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Density of human bone marrow stromal cells regulates commitment to vascular lineages
    Authors: Jemima L. Whyte, Stephen G. Ball, C. Adrian Shuttleworth, Keith Brennan, Cay M. Kielty
    Stem Cell Research
  13. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008-10-15;283(49):34250-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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