Human PILR-alpha Antibody

Catalog #: MAB64842 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB64842-100
MAB64842-SP

PILR-alpha binding to PANP blocked by PILR-alpha Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PILR-alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human PILR‑ alpha in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2175B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PILR‑ alpha with a C-terminal 6-His tag
Gln20-Thr196
Accession # Q9UKJ1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Neutralization

In a functional ELISA, 1-5 μg/mL of this antibody will block 50% of the binding of 2 μg/mL Recombinant Human PILR‑ alpha (Catalog # 6484-PR) to immobilized recombinant human PANP Fc Chimera coated at 5 μg/mL (100 µL/well).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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PILR-alpha binding to PANP blocked by PILR-alpha Antibody. In a functional ELISA, Recombinant Human PILR-a (Catalog # 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding.

Flow Cytometry

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Detection of PILR- alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human PILR- a or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PILR- a Monoclonal Antibody (Catalog # MAB64842) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14⁺CD1a^- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Long Name

Paired-Ig-likeType 2 Receptor alpha

Entrez Gene IDs

29992 (Human); 231805 (Mouse); 288568 (Rat)

Alternate Names

Cell surface receptor FDF03; FDF03; inhibitory receptor PILRalpha; Inhibitory receptor PILR-alpha; paired immunoglobin-like receptor alpha; paired immunoglobin-like type 2 receptor alpha; paired immunoglobulin-like receptor alpha; paired immunoglobulin-like type 2 receptor alpha; PILRA; PILRalpha; PILR-alpha