Human PILR-alpha PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: PILR-alpha

PILR-alpha (Paired Immunoglobulin-like, type 2 Receptor alpha) is a monomeric 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by neutrophils, monocytes, macrophages, CD14⁺CD1a^- DC, corneal epithelium and retinal pigment cells. It is known to bind to CD99, PANP, Collectin-12, and NPDC-1. The target for PILR-alpha appears to be sialylated O-glycans. PILR-alpha also acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and may either show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Product Datasheets

Related Research Areas

• Dendritic Cell ITIM/ITAM Immunoreceptors
• ITIM/ITAM Immunoreceptors and Related Molecules