Human PP4 Catalytic Subunit Antibody

Catalog #: MAB5074
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5074
MAB5074-SP

Detection of Human PP4 Catalytic Subunit by Western Blot.

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All PP4 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PP4 Catalytic Subunit Antibody Summary

Species Reactivity

Human

Specificity

Detects human, mouse, and rat PP4 Catalytic Subunit in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 501517

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PP4 Catalytic Subunit
Met1-Leu307
Accession # P60510

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human PP4 Catalytic Subunit by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PP4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP4 Catalytic Subunit at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP4

The catalytic subunit of Protein Phosphatase 4 (PP4), also called PPX, is a serine/threonine protein phosphatase with about 65% homology to PP2A. Although PP4 has the same heterotrimeric subunit structure as PP2A and can use many of the PP2A regulatory subunits, PP4 also has its own unique scaffolding and regulatory subunits. PP4 is largely found in the centrosome of the nucleus where it may play a role in modulating transcription factor phosphorylation by JNK. In Drosophila, cmm mutants have low levels of PP4 and are unable to complete mitosis due to a deficit in microtubule assembly.

Long Name

Protein Phosphatase 4

Entrez Gene IDs

5531 (Human); 56420 (Mouse); 171366 (Rat)

Alternate Names

EC 3.1.3.16; PP4; PP4C; PP4protein phosphatase X, catalytic subunit; PPH3; PPP4C; PPX; PP-X; PPXPPH3; protein phosphatase 4 (formerly X), catalytic subunit; protein phosphatase 4, catalytic subunit; Protein phosphatase X

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PP4 Catalytic Subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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