Human/Primate CCL5/RANTES Antibody Summary
Applications
Human/Primate CCL5/RANTES Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human CCL5/RANTES by Functional The expression of chemokine CCL5 is up-regulated in co-cultures of BMMSCs cells and OTSCC.Co-culture of OTSCC cells with BMMSCs results in the high expression of chemokine CCL5 in HSC-3 and SAS cells, but not in dysplastic DOK cells (A). Function-blocking monoclonal antibody against CCL5 (Mab CCL5) slightly inhibits BMMSC enhanced invasion area, but has no effect on the invasion depth, whereas the antibody against CXCL1 does not increase the inhibitory effect in OTSCC invasion (B, C). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24204919), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CCL5/RANTES by Immunocytochemistry/Immunofluorescence RANTES filaments are present after exposure to shear stress.(A) HUVECs were electroporated with DNA encoding RANTES, cultivated on coverslips before stimulation with TNF alpha + IFN gamma for 30 h. RANTESwas labeled with an anti-RANTES antibody (clone 21418), and the cover slipswere mounted in a laminar flow chamber. The flow rate was adjusted to mimicvessel wall shear stress of 1 dyne/cm2. The imageswere acquired by confocal microscopy before (left image, 0 min) andafter 4 min with exposure to flow forces (right image, 4 min).(B) HUVECs were treated as described in (A) except that they were notlabeled with anti-RANTES antibody before exposure to flow. Human peripheralblood mononuclear cells were labeled with an anti-CD45 antibody, resuspendedin medium and applied by a pump to the flow chamber(1 dyne/cm2). After 10 min, the cells werelabeled with a rabbit anti-RANTES antibody. Images were acquired bysequential scanning confocal microscopy. Arrows and arrow heads indicateRANTES filaments and RANTES positive platelets, respectively. Originalmagnification in all panels, × 100. Scale bars, 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25791723), licensed under a CC-BY license. Not internally tested by R&D Systems.
Neutralization by Human/Primate CCL5/RANTES Antibody. Human/Primate CCL5/RANTES Antibody (Catalog # MAB678) neutralizes Recombinant Human CCL5/RANTES (278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) for this effect is typically 80.0-800 ng/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.
Product Datasheets
Citations for Human/Primate CCL5/RANTES Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer
Authors: Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al.
Cancer Immunology Research
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Modulation of the CCR5 Receptor/Ligand Axis by Seminal Plasma and the Utility of In Vitro versus In Vivo Models
Authors: Jennifer A. Juno, Kathleen M. Wragg, Anne B. Kristensen, Wen Shi Lee, Kevin J. Selva, Renee M. van der Sluis et al.
Journal of Virology
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Activation of cGMP/PKG/p65 signaling associated with PDE5‐Is downregulates CCL5 secretion by CD8 + T cells in benign prostatic hyperplasia
Authors: Song Jin, Peng Xiang, Jie Liu, Yang Yang, Shuai Hu, Jindong Sheng et al.
The Prostate
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Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion
Authors: Yu Fan, Shuai Hu, Jie Liu, Fei Xiao, Xin Li, Wei Yu et al.
Mediators of Inflammation
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Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation
Authors: Rubina W. Saeed, Santosh Varma, Tina Peng-Nemeroff, Barbara Sherry, David Balakhaneh, Jared Huston et al.
The Journal of Experimental Medicine
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Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells.
Authors: Oynebraten I, Barois N, Bergeland T, Kuchler A, Bakke O, Haraldsen G
Sci Rep, 2015-03-20;5(0):9261.
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A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris
Authors: Fabrice Cerini, Hubert Gaertner, Knut Madden, Ilya Tolstorukov, Scott Brown, Bram Laukens et al.
Protein Expression and Purification
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Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study
Authors: S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho
Scientific Reports, 2022-12-15;12(1):21667.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Cancer-associated fibroblast-derived CCL5 contributes to cisplatin resistance in A549 NSCLC cells partially through upregulation of lncRNA HOTAIR expression
Authors: X Sun, Z Chen
Oncology Letters, 2021-08-02;22(4):696.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
Nature Communications, 2021-07-02;12(1):4091.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
Cancers, 2021-03-16;13(6):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers
Authors: PS Arunachala, TP Charles, V Joag, VS Bollimpell, MKD Scott, F Wimmers, SL Burton, CC Labranche, C Petitdeman, S Gangadhara, TM Styles, CF Quarnstrom, KA Walter, TJ Ketas, T Legere, PB Jagadeesh, SP Kasturi, A Tsai, BZ Yeung, S Gupta, M Tomai, J Vasilakos, GM Shaw, CY Kang, JP Moore, S Subramania, P Khatri, D Montefiori, PA Kozlowski, CA Derdeyn, E Hunter, D Masopust, RR Amara, B Pulendran
Nat. Med., 2020-05-11;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
DSE regulates the malignant characters of hepatocellular carcinoma cells by modulating CCL5/CCR1 axis
Authors: WC Liao, HR Yen, CK Liao, TJ Tseng, CT Lan, CH Liu
Am J Cancer Res, 2019-02-01;9(2):347-362.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
PLoS Pathog., 2018-02-23;14(2):e1006888.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
Cancer Sci., 2018-02-14;109(3):724-731.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways
Authors: YL Zhang, Q Li, XM Yang, F Fang, J Li, YH Wang, Q Yang, L Zhu, HZ Nie, XL Zhang, MX Feng, SH Jiang, GA Tian, LP Hu, HY Lee, SJ Lee, Q Xia, ZG Zhang
Cancer Res., 2018-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/?-catenin signaling through stabilization of the WNT-receptor complex
Authors: SJ Zhao, YQ Jiang, NW Xu, Q Li, Q Zhang, SY Wang, J Li, YH Wang, YL Zhang, SH Jiang, YJ Wang, YJ Huang, XX Zhang, GA Tian, CC Zhang, YY Lv, M Dai, F Liu, R Zhang, D Zhou, ZG Zhang
Oncogene, 2017-10-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
Sci Rep, 2017-02-20;7(0):42893.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
Authors: CM Henry, SJ Martin
Mol. Cell, 2017-02-16;65(4):715-729.e5.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Authors: Osei-Kumah A, Smith R, Clifton VL
Cytokine, 2008-07-17;43(2):187-93.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
Clin. Immunol., 2008-04-22;128(1):57-65.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008-02-15;122(4):769-76.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
Clin. Chem., 2007-11-01;53(11):2010-2.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007-09-21;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS, 2007;21(10):1263-72.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
Int. Immunol., 2006-06-01;18(8):1243-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Mass Spectometry -
Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
Immunol. Lett., 2006-03-03;105(2):159-66.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
Authors: Edwards MR, Johnson MW, Johnston SL
Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
J. Virol., 2006-01-01;80(1):236-45.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002-11-01;169(9):5244-51.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Authors: Hirst SJ, Hallsworth MP, Peng Q
Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
Immunity, 2001-06-01;14(6):705-14.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Chemoattractant factors in breast milk from allergic and nonallergic mothers.
Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
Pediatr. Res., 2000-05-01;47(5):592-7.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity
Authors: Peijian Zhang, Erlinda The, Zichao Luo, Yufeng Zhai, Qingzhou Yao, Lihua Ao et al.
Molecular Medicine
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Human Bone Marrow Mesenchymal Stem Cells Induce Collagen Production and Tongue Cancer Invasion
Authors: Sirpa Salo, Carolina Bitu, Kalle Merkku, Pia Nyberg, Ibrahim O Bello, Jussi Vuoristo et al.
PLoS ONE
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Used as a capture in our NHP ELISA