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Human/Primate EGF Antibody

Catalog #: MAB636
7 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB636-SP
MAB636-100
MAB636-500
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EGF in Human Lung Cancer Tissue.
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Product Details
Citations (7)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate EGF Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate EGF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference is observed with recombinant mouse (rm) EGF, bovine FGF acidic, bovine FGF basic, recombinant human (rh) G-CSF, rhGM-CSF, rmGM-CSF, rhLIF, human PDGF, porcine PDGF, rhTGF‑ alpha, hTGF-beta 1, pTGF-beta 1, rhTGF-beta 1, porcine TGF-beta 1.2, porcine TGF-beta 2, rhTNF-alpha, or rhTNF-beta.
Source
Monoclonal Mouse IgG1 Clone # 10827
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human EGF
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Human/Primate EGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate EGF Biotinylated Antibody (Catalog # BAF236)

Standard: Recombinant Human EGF Protein, CF (Catalog # 236-EG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry EGF antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

EGF in Human Lung Cancer Tissue. EGF was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human/Primate EGF Monoclonal Antibody (Catalog # MAB636) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membrane and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name
Epidermal Growth Factor
Entrez Gene IDs
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

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Citations for Human/Primate EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Synergism between Hedgehog-GLI and EGFR Signaling in Hedgehog-Responsive Human Medulloblastoma Cells Induces Downregulation of Canonical Hedgehog-Target Genes and Stabilized Expression of GLI1
    Authors: Frank Götschel, Daniela Berg, Wolfgang Gruber, Christian Bender, Markus Eberl, Myriam Friedel et al.
    PLoS ONE
  2. Leptin receptor expression and its change in association with the normalization of EGF profile after seminal plasma treatment in repeat breeder dairy cows
    Authors: Nattapong NINPETCH, Dagvajamts BADRAKH, HAY MAR KYAW, Kohei KAWANO, Yojiro YANAGAWA, Masashi NAGANO et al.
    Journal of Reproduction and Development
  3. Effects of heat stress on the endometrial epidermal growth factor profile and fertility in dairy cows
    Authors: Kohei KAWANO, Yojiro YANAGAWA, Masashi NAGANO, Seiji KATAGIRI
    Journal of Reproduction and Development
  4. Effects of recombinant osteopontin expressed in Escherichia coli on the recovery of the endometrial epidermal growth factor profile and fertility in repeat breeder dairy cows
    Authors: Tanida, T;Tagami, T;Sato, H;Kyaw, HM;Fujikawa, T;Nagano, M;Momozawa, K;Yanagawa, Y;Katagiri, S;
    Theriogenology
    Species: Bovine
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  5. Effects of prostaglandin F2? treatment at follicular wave emergence on endometrial epidermal growth factor concentration on day 3 of the next estrous cycle and on fertility in dairy cows
    Authors: Phong, V;Fujikawa, T;Kawano, K;Yanagawa, Y;Katagiri, S;
    Theriogenology
    Species: Bovine
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  6. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012-02-17;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  7. Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light.
    Authors: Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F
    J. Biotechnol., 2006-04-03;125(2):159-72.
    Species: Human
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: ELISA Development, Western Blot

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Reviews for Human/Primate EGF Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Primate EGF Antibody
By Anonymous on 07/19/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human
ELISA Human/Primate EGF Antibody MAB636
Human/Primate EGF Antibody
By Anonymous on 11/07/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used to develop a sandwich ELISA for the measurement of EGF in human serum. It worked very well using AF236 as a matched pair.

ELISA Human/Primate EGF Antibody MAB636