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Human/Primate MMP-9 Antibody

Catalog #: MAB936
15 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB936-SP
MAB936-500
MAB936-100
MMP‑9 in SW1353 Human Cell Line.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate MMP-9 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human MMP-9 in Western blots. Does not react with the C-terminal truncated form (65 kDa) of recombinant human (rh) MMP-9. In Western blots, approximately 5% cross-reactivity with rhMMP-2 and no cross-reactivity with rhMMP-1 or rhMMP-3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 36020
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑9 Western Blot Standard (Catalog # WBC018)
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
Immunocytochemistry
3-25 µg/mL
See below

Human/Primate MMP-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate MMP‑9 Biotinylated Antibody (Catalog # BAF911)

Standard: Recombinant Human MMP-9 Protein, CF (Catalog # 911-MP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MMP-9 antibody in SW1353 Human Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑9 in SW1353 Human Cell Line. MMP-9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP-9 antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

MMP‑9 in Human Lung. MMP-9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Pro Matrix Metalloproteinase 9 (Pro-MMP-9) is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9, which can degrade collagens and elastin as well as several non-ECM molecules.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

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Citations for Human/Primate MMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. The Elevated Inflammatory Status of Neutrophils Is Related to In-Hospital Complications in Patients with Acute Coronary Syndrome and Has Important Prognosis Value for Diabetic Patients
    Authors: Barbu, E;Mihaila, AC;Gan, AM;Ciortan, L;Macarie, RD;Tucureanu, MM;Filippi, A;Stoenescu, AI;Petrea, SV;Simionescu, M;Balanescu, SM;Butoi, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. The Significance of Matrix Metalloproteinase 9 (MMP-9) and Metalloproteinase 2 (MMP-2) in Urinary Bladder Cancer
    Authors: J Kudelski, A Tokarzewic, M Gudowska-S, B Mroczko, P Ch?osta, M Bruczko-Go, P Mitura, G M?ynarczyk
    Biomedicines, 2023-03-20;11(3):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Characterization of Active MMP9 in Chronic Inflammatory Diseases Using a Novel Anti-MMP9 Antibody
    Authors: Maile Velasquez, Chris O’Sullivan, Robert Brockett, Amanda Mikels-Vigdal, Igor Mikaelian, Victoria Smith et al.
    Antibodies (Basel)
  4. Long-term and potent IOP-lowering effect of I kappa B alpha -siRNA in a nonhuman primate model of chronic ocular hypertension
    Authors: Difang Sun, Zongyi Zhan, Rui Zeng, Xiaolin Liu, Bin Wang, Fan Yang et al.
    iScience
  5. Relevance of Plasma Matrix Metalloproteinase-9 for Bronchiolitis Obliterans Syndrome after Allogeneic Hematopoietic Cell Transplantation
    Authors: Yoshihiro Inamoto, Paul J. Martin, Lynn E. Onstad, Guang-Shing Cheng, Kirsten M. Williams, Iskra Pusic et al.
    Transplantation and Cellular Therapy
  6. Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking
    Authors: W Na, EJ Lee, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, YH Kang
    Int J Mol Sci, 2020-11-13;21(22):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Low Plasma IL-8 Levels During Chemotherapy Are Predictive of Excellent Long-Term Survival in Metastatic Breast Cancer
    Authors: L Tiainen, M Hämäläinen, T Luukkaala, M Tanner, O Lahdenperä, P Vihinen, A Jukkola, P Karihtala, E Moilanen, PL Kellokumpu
    Clin. Breast Cancer, 2019-04-04;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  8. Blockade of monocyte-endothelial trafficking by transduced Tat-superoxide dismutase protein
    Authors: SIN-HYE PARK, MIN JAE SHIN, DAE WON KIM, JINSEU PARK, SOO YOUNG CHOI, YOUNG-HEE KANG
    International Journal of Molecular Medicine
  9. Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies.
    Authors: Ohlsson S, Ohlsson S, Soderberg D, Gunnarsson L, Pettersson A, Segelmark M, Hellmark T
    Clin Exp Immunol, 2014-06-01;176(3):363-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  10. Expression of matrix metalloproteinases 2, 7 and 9 in patients with colorectal cancer
    Authors: Elena Kostova, Maja Slaninka-Miceska, Nikola Labacevski, Krume Jakovski, Jasmina Trojachanec, Emilija Atanasovska et al.
    Vojnosanitetski pregled
  11. In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
    Authors: Schneider P, Costa O, Legrand E
    Leuk. Res., 2009-09-12;34(1):24-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
    Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
    Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Distinct synovial immunopathologic characteristics of juvenile-onset spondylarthritis and other forms of juvenile idiopathic arthritis.
    Authors: Kruithof E, Van den Bossche V, De Rycke L, Vandooren B, Joos R, Canete JD, Tak PP, Boots AM, Veys EM, Baeten D
    Arthritis Rheum., 2006-08-01;54(8):2594-604.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.
    Authors: Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C
    Laryngoscope, 2005-01-01;115(1):56-61.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Increased production of matrix metalloproteinases in Helicobacter pylori-associated human gastritis.
    Authors: Bergin PJ, Anders E, Sicheng W, Erik J, Jennie A, Hans L, Pierre M, Qiang PH, Marianne QJ
    Helicobacter, 2004-06-01;9(0):201.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human/Primate MMP-9 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
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Human/Primate MMP-9 Antibody
By Anonymous on 10/04/2021
Application: IHC Sample Tested: Squamous cell carcinoma Species: Human
Immunohistochemistry Human/Primate MMP-9 Antibody MAB936
Human/Primate MMP-9 Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

293T cells were infected by lentivirus overexpression of control or MMP-9 for 72h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human MMP-9 Antibody (MAB936). A specific band was detected for MMP-9 at approximately 74 kDa. This experiment was conducted under reducing conditions

Western Blot Human/Primate MMP-9 Antibody MAB936
Human/Primate MMP-9 Antibody
By Anonymous on 11/10/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

A sandwich ELISA was built using this antibody as the capture and AF911 as the detection antibody. MMP-9 was measured in human serum and plasma samples.

ELISA Human/Primate MMP-9 Antibody MAB936