Human Pro-Collagen I alpha 1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: Collagen I alpha 1
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.
Citations for Human Pro-Collagen I alpha 1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Wrinkle-Improving Effect of Novel Peptide That Binds to Nicotinic Acetylcholine Receptor
Authors: Bang, J;Hwang, YL;Kim, MY;Yun, JN;Hyun, E;Chang, MY;Shin, DH;Kim, S;Lee, JH;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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Oxidized Phospholipids Regulate Tenocyte Function via Induction of Amphiregulin in Dendritic Cells
Authors: Pinnarò, V;Kirchberger, S;Künig, S;Gil Cantero, S;Ciardulli, MC;Della Porta, G;Blüml, S;Elbe-Bürger, A;Bochkov, V;Stöckl, J;
International journal of molecular sciences
Species: Human
Sample Types: Complex Sample Type
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A metabolic-dysfunction associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system
Authors: Aleman, J;Ravikumar, K;Wiegand, C;Schurdak, ME;Vernetti, L;Gavlock, D;Reese, C;DeBiasio, R;LaRocca, G;Angarita, YD;Gough, A;Soto-Gutierrez, A;Behari, J;Yechoor, V;Miedel, MT;Stern, AM;Banerjee, I;Taylor, DL;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Tissue Homogenates
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Pro-Resolving Mediators in Rotator Cuff Disease: How Is the Bursa Involved?
Authors: Klatte-Schulz, F;Bormann, N;Bonell, A;Al-Michref, J;Nguyen, HL;Klöckner, P;Thiele, K;Moroder, P;Seifert, M;Sawitzki, B;Wildemann, B;Duda, GN;
Cells
Species: Human
Sample Types: Cell Culture Supernates
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Human Probiotic Lactobacillus paracasei-Derived Extracellular Vesicles Improve Tumor Necrosis Factor-?-Induced Inflammatory Phenotypes in Human Skin
Authors: Lee, KS;Kim, Y;Lee, JH;Shon, S;Kim, A;Pham, AVQ;Kim, C;Kim, DH;Kim, YK;Cho, EG;
Cells
Species: Human
Sample Types: Cell Culture Supernates
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Anti-Aging and Depigmentation Effect of a Hyaluronic Acid Mechanically Stabilized Complex on Human Skin Explants
Authors: Siquier-Dameto, G;Boisnic, S;Boadas-Vaello, P;Verdú, E;
Polymers
Species: Human
Sample Types: Cell Lysates
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The Role of CTGF in Liver Fibrosis Induced in 3D Human Liver Spheroids
Authors: SR Trampuž, S van Riet, Å Nordling, M Ingelman-S
Cells, 2023-01-13;12(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Enhancement of Efficacy of Retinoids through Enhancing Retinoid-Induced RAR Activity and Inhibiting Hydroxylation of Retinoic Acid, and Its Clinical Efficacy on Photo-Aging
Authors: S Kang, H Lee, SH Jun, SG Park, NG Kang
Pharmaceutics, 2022-11-08;14(11):.
Species: Human
Sample Types: Cell Culture Supernates
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IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
Int J Mol Sci, 2022-08-29;23(17):.
Species: Human
Sample Types: Tissue Culture Supernates
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Knockout and Double Knockout of Cathepsin K and Mmp9 reveals a novel function of Cathepsin K as a regulator of osteoclast gene expression and bone homeostasis
Authors: G Zhu, W Chen, CY Tang, A McVicar, D Edwards, J Wang, M McConnell, S Yang, Y Li, Z Chang, YP Li
International journal of biological sciences, 2022-08-29;18(14):5522-5538.
Species: Mouse
Sample Types: Serum
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Biological Impact of gamma-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications
Authors: FA Fadeyev, FA Blyakhman, AP Safronov, GY Melnikov, AD Nikanorova, IP Novoselova, GV Kurlyandsk
Biosensors, 2022-08-11;12(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Association of bone-related�biomarkers with femoral neck bone strength
Authors: N Xia, Y Cai, W Wang, C Bao, Y Li, Q Xie, W Xu, D Liu
Bmc Musculoskeletal Disorders, 2022-05-21;23(1):482.
Species: Human
Sample Types: Serum
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Peri-implant cell response on groove and pore-textured zirconia surfaces
Authors: MB da Cruz, JF Marques, BF Fernandes, S Madeira, Ó Carvalho, FS Silva, ADSP da Mata, JMM Caramês
Journal of oral biosciences, 2022-02-07;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Genome-wide chromatin contacts of super-enhancer-associated lncRNA identify LINC01013 as a regulator of fibrosis in the aortic valve
Authors: A Chignon, D Argaud, MC Boulanger, G Mkannez, V Bon-Baret, Z Li, S Thériault, Y Bossé, P Mathieu
PloS Genetics, 2022-01-18;18(1):e1010010.
Species: Human
Sample Types: Cell Culture Supernates
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Antifibrotic effect of brown algae-derived fucoidans on osteoarthritic fibroblast-like synoviocytes
Authors: M Piñeiro-Ra, N Flórez-Fer, O Ramil-Góme, MD Torres, H Dominguez, FJ Blanco, R Meijide-Fa, C Vaamonde-G
Carbohydrate polymers, 2022-01-12;282(0):119134.
Species: Human
Sample Types: Cell Culture Supernates
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Molecular Weight Analysis of Blue Shark (Prionace glauca) Collagen Hydrolysates by GPC-LS; Effect of High Molecular Weight Hydrolysates on Fibroblast Cultures: mRNA Collagen Type I Expression and Synthesis
Authors: M Blanco, N Sanz, AC Sánzhez, B Correa, RI Pérez-Mart, CG Sotelo
International Journal of Molecular Sciences, 2021-12-21;23(1):.
Species: Human
Sample Types: Cell Culture Supernates
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The serine proteases dipeptidyl-peptidase 4 and urokinase are key molecules in human and mouse scar formation
Authors: V Vorstandle, M Laggner, D Copic, K Klas, M Direder, Y Chen, B Golabi, W Haslik, C Radtke, E Tschachler, K Hötzenecke, HJ Ankersmit, M Mildner
Nature Communications, 2021-10-29;12(1):6242.
Species: Human
Sample Types: Cell Culture Supernates
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A mouse model of brittle cornea syndrome caused by mutation in Zfp469
Authors: CM Stanton, AS Findlay, C Drake, MZ Mustafa, P Gautier, L McKie, IJ Jackson, V Vitart
Disease Models & Mechanisms, 2021-09-22;14(9):.
Species: Mouse, Transgenic Mouse
Sample Types: Cell Culture Supernates
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Serum levels of irisin in postmenopausal women with osteoporotic hip fractures
Authors: K Liu, P Jing, Z Liu, Y Wang, Z Han, Y Wang, Z Zheng, Y Wu, T Wang, Y Li, H Zhang, L Wang
Cytokine, 2021-09-21;148(0):155708.
Species: Human
Sample Types: Serum
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WNT/beta-Catenin Signaling Promotes TGF-beta-Mediated Activation of Human Cardiac Fibroblasts by Enhancing IL-11 Production
Authors: E Dzia?o, M Czepiel, K Tkacz, M Siedlar, G Kania, P B?yszczuk
International Journal of Molecular Sciences, 2021-09-17;22(18):.
Species: Human
Sample Types: Cell Culture Supernates
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Activation of NF-&kapppaB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion
Authors: X Zhou, LJ Backman, P Danielson
Scientific Reports, 2021-04-01;11(1):7360.
Species: Human
Sample Types: Cell Culture Supernates
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MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway
Authors: Y Xia, L Zhen, H Li, S Wang, S Chen, C Wang, X Yang
Cell Death & Disease, 2021-03-26;12(4):326.
Species: Human
Sample Types: Cell Culture Supernates
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Platelet-Released Growth Factors and Platelet-Rich Fibrin Induce Expression of Factors Involved in Extracellular Matrix Organization in Human Keratinocytes 22.39
Authors: A Bayer, B Wijaya, L Möbus, F Rademacher, M Rodewald, M Tohidnezha, T Pufe, D Drücke, R Gläser, J Harder
Int J Mol Sci, 2020-06-20;21(12):.
Species: Human
Sample Types: Cell Culture Supernates
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Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis
Authors: B Baeza-Raja, A Goodyear, X Liu, K Lam, L Yamamoto, Y Li, GS Dodson, T Takeuchi, T Kisseleva, DA Brenner, K Dabbagh
PLoS ONE, 2020-06-03;15(6):e0234038.
Species: Human
Sample Types: Cell Culture Supernates
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Syringaresinol Inhibits UVA-Induced MMP-1 Expression by Suppression of MAPK/AP-1 Signaling in HaCaT Keratinocytes and Human Dermal Fibroblasts
Authors: JH Oh, YH Joo, F Karadeniz, J Ko, CS Kong
Int J Mol Sci, 2020-06-01;21(11):.
Species: Human
Sample Types: Cell Culture Supernates
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Activated Cardiac Fibroblasts Control Contraction of Human Fibrotic Cardiac Microtissues by a beta-Adrenoreceptor-Dependent Mechanism
Authors: P B?yszczuk, C Zuppinger, A Costa, D Nurzynska, FD Di Meglio, M Stellato, I Agarkova, GL Smith, O Distler, G Kania
Cells, 2020-05-20;9(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Predictive Value of Precision-Cut Kidney Slices as an Ex Vivo Screening Platform for Therapeutics in Human Renal Fibrosis
Authors: E Bigaeva, N Puerta Cav, EGD Stribos, AJ de Jong, C Biel, HAM Mutsaers, MS Jensen, R Nørregaard, AM Leliveld, IJ de Jong, JL Hillebrand, H van Goor, M Boersema, RA Bank, P Olinga
Pharmaceutics, 2020-05-18;12(5):.
Species: Human
Sample Types: Tissue Culture Supernates
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Anticatabolic and Anti-Inflammatory Effects of Myricetin 3-O-&beta-d-Galactopyranoside in UVA-Irradiated Dermal Cells via Repression of MAPK/AP-1 and Activation of TGF&beta/Smad
Authors: JH Oh, F Karadeniz, JI Lee, SY Park, Y Seo, CS Kong
Molecules, 2020-03-14;25(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Proton Pump Inhibitors Reduce Pancreatic Adenocarcinoma Progression by Selectively Targeting H+, K+-ATPases in Pancreatic Cancer and Stellate Cells
Authors: M Tozzi, CE Sørensen, L Magni, NM Christense, R Bouazzi, CM Buch, M Stefanini, C Duranti, A Arcangeli, I Novak
Cancers (Basel), 2020-03-10;12(3):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds
Authors: P Zhou, G Chu, Z Yuan, H Wang, W Zhang, Y Mao, X Zhu, W Chen, H Yang, B Li
Journal of Orthopaedic Translation, 2020-03-03;26(0):171-180.
Species: Rabbit
Sample Types: Cell Culture Supernates
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Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P
Authors: HY Lim, D Jeong, SH Park, KK Shin, YH Hong, E Kim, YG Yu, TR Kim, H Kim, J Lee, JY Cho
Int J Mol Sci, 2020-02-27;21(5):.
Species: Human
Sample Types: Cell Culture Supernates
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&alphaAzithromycin has enhanced effects on lung fibroblasts from idiopathic pulmonary fibrosis (IPF) patients compared to controls
Authors: K Krempaska, S Barnowski, J Gavini, N Hobi, S Ebener, C Simillion, A Stokes, R Schliep, L Knudsen, TK Geiser, M Funke-Cham
Respir. Res., 2020-01-15;21(1):25.
Species: Human
Sample Types: Cell Culture Superantes
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Antiphotoaging effect of boiled abalone residual peptide ATPGDEG on UVB-induced keratinocyte HaCaT cells
Authors: J Chen, P Liang, Z Xiao, MF Chen, F Gong, C Li, C Zhou, P Hong, WK Jung, ZJ Qian
Food Nutr Res, 2019-11-08;63(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Hydrangenol Isolated from the Leaves of Hydrangea serrata Attenuates Wrinkle Formation and Repairs Skin Moisture in UVB-Irradiated Hairless Mice
Authors: DB Myung, HS Han, JS Shin, JY Park, HJ Hwang, HJ Kim, HS Ahn, SH Lee, KT Lee
Nutrients, 2019-10-02;11(10):.
Species: Mouse
Sample Types: Tissue Homogenates
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Attenuation of diabetic cardiomyopathy by relying on kirenol to suppress inflammation in a diabetic rat model
Authors: B Wu, XY Huang, L Li, XH Fan, PC Li, CQ Huang, J Xiao, R Gui, S Wang
J. Cell. Mol. Med., 2019-09-29;0(0):.
Species: Rat
Sample Types: Cell Culture Supernates
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Primary Human Fibroblasts in Culture Switch to a Myofibroblast-Like Phenotype Independently of TGF Beta
Authors: U Baranyi, B Winter, A Gugerell, B Hegedus, C Brostjan, G Laufer, B Messner
Cells, 2019-07-13;8(7):.
Species: Human
Sample Types: Cell Culture Supernates
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Probucol ameliorates EMT and lung fibrosis through restoration of SIRT3 expression
Authors: HX Zhang, YN Li, XL Wang, CL Ye, XY Zhu, HP Li, T Yang, YJ Liu
Pulm Pharmacol Ther, 2019-05-11;0(0):101803.
Species: Mouse
Sample Types: Tissue Homogenates
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WNT3a and WNT5a Transported by Exosomes Activate WNT Signaling Pathways in Human Cardiac Fibroblasts
Authors: E Dzia?o, M Rudnik, RI Koning, M Czepiel, K Tkacz, M Baj-Krzywo, O Distler, M Siedlar, G Kania, P B?yszczuk
Int J Mol Sci, 2019-03-21;20(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Gender-independent efficacy of mesenchymal stem cell therapy in sex hormone-deficient bone loss via immunosuppression and resident stem cell recovery
Authors: BD Sui, J Chen, XY Zhang, T He, P Zhao, CX Zheng, M Li, CH Hu, Y Jin
Exp. Mol. Med., 2018-12-17;50(12):166.
Species: Human
Sample Types: Serum
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microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A
Authors: RH Qin, H Tao, SH Ni, P Shi, C Dai, KH Shi
Anatol J Cardiol, 2018-10-01;20(4):198-205.
Species: Rat
Sample Types: Serum
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Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
Authors: E Raschi, CB Chighizola, L Cesana, D Privitera, F Ingegnoli, C Mastaglio, PL Meroni, MO Borghi
Arthritis Res. Ther., 2018-08-29;20(1):187.
Species: Human
Sample Types: Cell Culture Supernates
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Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression
Authors: AR Han, TG Lim, YR Song, M Jang, YK Rhee, HD Hong, MH Kim, HJ Kim, CW Cho
Int J Mol Sci, 2018-08-24;19(9):.
Species: Human
Sample Types: Cell Culture Supernates
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Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking
Authors: Y Kondo, J Fu, H Wang, C Hoover, JM McDaniel, R Steet, D Patra, J Song, L Pollard, S Cathey, T Yago, G Wiley, S Macwana, J Guthridge, S McGee, S Li, C Griffin, K Furukawa, JA James, C Ruan, RP McEver, KJ Wierenga, PM Gaffney, L Xia
JCI Insight, 2018-07-26;3(14):.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-Tumour Necrosis Factor Therapy for Dupuytren's Disease: A Randomised Dose Response Proof of Concept Phase 2a Clinical Trial
Authors: J Nanchahal, C Ball, D Davidson, L Williams, W Sones, FE McCann, M Cabrita, J Swettenham, NJ Cahoon, B Copsey, E Anne Franc, PC Taylor, J Black, VS Barber, S Dutton, M Feldmann, SE Lamb
EBioMedicine, 2018-07-06;0(0):.
Species: Human
Sample Types: Serum
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Protective Effects of LSGYGP from Fish Skin Gelatin Hydrolysates on UVB-Induced MEFs by Regulation of Oxidative Stress and Matrix Metalloproteinase Activity
Authors: Q Ma, Q Liu, L Yuan, Y Zhuang
Nutrients, 2018-03-28;10(4):.
Species: Mouse
Sample Types: Cell Culture Supernates
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The Potential Role of Bile Acids in Acquired Laryngotracheal Stenosis
Authors: A Aldhahrani, J Powell, S Ladak, M Ali, S Ali, B Verdon, J Pearson, C Ward
Laryngoscope, 2018-02-05;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders
Authors: A Beierfu beta, H Dietrich, C Kremser, M Hunjadi, A Ritsch, T Rülicke, C Thomé, DS Mern
PLoS ONE, 2017-11-03;12(11):e0187564.
Species: Rabbit
Sample Types: Tissue Homogenates
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Differential Proteomics Identifies Reticulocalbin-3 as a Novel Negative Mediator of Collagen Production in Human Cardiac Fibroblasts
Authors: E Martínez-M, J Ibarrola, A Fernández-, E Santamaria, J Fernández-, P Rossignol, F Jaisser, N López-Andr
Sci Rep, 2017-09-22;7(1):12192.
Species: Human
Sample Types: Cell Culture Supernates
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Dynamin-Related Protein 1 Inhibition Attenuates Cardiovascular Calcification in the Presence of Oxidative Stress
Authors: M Rogers, N Maldonado-, JD Hutcheson, C Goettsch, S Goto, I Yamada, T Faits, H Sesaki, M Aikawa, E Aikawa
Circ. Res., 2017-06-12;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Asiaticoside hinders the invasive growth of keloid fibroblasts through inhibition of the GDF-9/MAPK/Smad pathway
Authors: X Wu, D Bian, Y Dou, Z Gong, Q Tan, Y Xia, Y Dai
J. Biochem. Mol. Toxicol, 2017-03-27;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis
JCI Insight, 2016-12-08;1(20):e90954.
Species: Human
Sample Types: Cell Culture Supernates
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Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
Cancer Res., 2016-11-18;0(0):.
Species: Human
Sample Types: Tissue Homogenates
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Characterization of the MMP/TIMP Imbalance and Collagen Production Induced by IL-1? or TNF-? Release from Human Hepatic Stellate Cells
Authors: S Robert, T Gicquel, A Bodin, V Lagente, E Boichot
PLoS ONE, 2016-04-05;11(4):e0153118.
Species: Human
Sample Types: Cell Culture Supernates
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Angiotensin-(1-7) and angiotensin induce the transdifferentiation of human endometrial epithelial cells in vitro.
Authors: Shan T, Zhang L, Zhao C, Chen W, Zhang Y, Li G
Mol Med Rep, 2014-04-09;9(6):2180-6.
Species: Human
Sample Types: Cell Culture Supernates
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The atherogenic Scarb1 null mouse model shows a high bone mass phenotype.
Authors: Martineau C, Martin-Falstrault L, Brissette L, Moreau R
Am J Physiol Endocrinol Metab, 2013-11-19;306(1):E48-57.
Species: Mouse
Sample Types: Plasma
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Dermal fibroblast cell culture supernatant following stimulation with 2ng/mL TGFb1
using supernatants from primary lung fibroblasts