Human Pro-Gastrin-releasing Peptide DuoSet ELISA

Catalog # Availability Size / Price Qty
DY7847-05
Ancillary Products Available
Human Gastrin-releasing Peptide / Bombesin ELISA Standard Curve
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human Pro-Gastrin-releasing Peptide DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
9.4 - 600 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Gastrin-releasing Peptide / Bombesin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Gastrin-releasing Peptide / Bombesin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citation for Human Pro-Gastrin-releasing Peptide DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Postprandial effect of gastrointestinal hormones and gastric activity in patients with irritable bowel syndrome
    Authors: Furga?a, A;Ciesielczyk, K;Przybylska-Felu?, M;Jab?o?ski, K;Gil, K;Zwoli?ska-Wcis?o, M;
    Scientific reports
    Species: Human
    Sample Types: Plasma

FAQs

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Reviews for Human Pro-Gastrin-releasing Peptide DuoSet ELISA

Average Rating: 3 (Based on 1 Review)

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Human Pro-Gastrin-releasing Peptide DuoSet ELISA
By Anonymous on 08/17/2018
Sample Tested: EDTA Plasma