Home / MMP-9 / Human Pro MMP-9 Antibody

Human Pro MMP-9 Antibody

Catalog #: MAB9111
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9111
MAB9111-SP
Detection of Human Pro MMP‑9 by Western Blot.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pro MMP-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pro MMP-9 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, 2, 3, 7, 8, 10, 12, 13, 14, 16, or recombinant mouse MMP-9 is observed.
Source
Monoclonal Mouse IgG2A Clone # 526515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑9
Ala20-Asp707 (Gln279Arg)
Accession # P14780
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Pro MMP‑9 antibody by Western Blot. View Larger

Detection of Human Pro MMP‑9 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line stimulated with 100 ng/ml PMA for 24 hours under reducing (R) and non-reducing (NR) conditions. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Pro MMP-9 Monoclonal Antibody (Catalog # MAB9111) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Pro MMP-9 was detected at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (Gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Pro MMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Minocycline inhibits PDGF-BB-induced human aortic smooth muscle cell proliferation and migration by reversing miR-221- and -222-mediated RECK suppression
    Authors: Y Higashi, S Mummidi, S Sukhanov, T Yoshida, M Noda, P Delafontai, B Chandrasek
    Cell. Signal., 2019-02-01;57(0):20-Oct.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Matrix-metalloproteinase-9 is cleaved and activated by Cathepsin K
    Authors: Jon Christensen, V Prasad Shastri
    BMC Research Notes
  3. Deferoxamine Treatment Improves Antioxidant Cosmeceutical Formulation Protection against Cutaneous Diesel Engine Exhaust Exposure
    Authors: Pambianchi E, Ferrara F, Pecorelli A et al.
    Antioxidants (Basel, Switzerland)

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Pro MMP-9 Antibody

There are currently no reviews for this product. Be the first to review Human Pro MMP-9 Antibody and earn rewards!

Have you used Human Pro MMP-9 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review