Human Profilin 1 Antibody Summary
Met1-Tyr140
Accession # P07737
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Profilin 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Profilin 1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Profilin 1 by Simple WesternTM. Simple Western shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Profilin 1 at approximately 18 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Profilin 1
Profilin 1 (gene name PFN1) is a ubiquitous, cytoplasmic, 140 amino acid, 12-15 kDa actin monomer-binding protein. It facilitates adenine nucleotide exchange, supplying ATP for actin filament polymerization. It is phosphorylated and acetylated, and binds proline-rich sequences. Human Profilin 1 shares 96% amino acid sequence identity with mouse and rat Profilin 1.
Product Datasheets
Citations for Human Profilin 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Serum proteomic identification and validation of two novel atherosclerotic aortic aneurysm biomarkers, profilin 1 and complement factor D
Authors: Yusuke Murakami, Mitsuhiro Nishigori, Hiroaki Yagi, Tsukasa Osaki, Masaki Wakabayashi, Manabu Shirai et al.
Proteome Science
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The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence
Authors: Ayoubi, R;McDowell, I;Fotouhi, M;Southern, K;McPherson, PS;Laflamme, C;NeuroSGC/YCharOS/EDDU collaborative group, ;ABIF Consortium, ;
F1000Research
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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